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Literature summary for 1.14.16.2 extracted from

  • Nelson, T.J.; Kaufman, S.
    Interaction of tyrosine hydroxylase with ribonucleic acid and purification with DNA-cellulose or poly(A)-sepharose affinity chromatography (1987), Arch. Biochem. Biophys., 257, 69-84.
    View publication on PubMed
Search for more literature for 1.14.16.2

Activating Compound

Activating Compound Comment Organism Structure
RNA below 0.015 mg/ml activation, rat brain enzyme contains RNA, about 10% of the total mass Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
RNA above 0.015 mg/ml Bos taurus
RNA above 0.015 mg/ml Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of RNA on Km Rattus norvegicus
additional information
-
 additional information effect of RNA on Km Bos taurus
additional information
-
 additional information rat brain: 2 kinetically distinguishable forms: low Km form, high Km form Rattus norvegicus
0.0061
-
 tyrosine
-
 Rattus norvegicus
0.08
-
 Tetrahydropterin low Km form Rattus norvegicus
0.63
-
 Tetrahydropterin high Km form Rattus norvegicus
0.95
-
 6-Methyl-5,6,7,8-tetrahydropterin
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
phosphate
-
 Bos taurus
phosphate activated enzyme contains: 0.75 mol per mol of subunit Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61100
-
 2 * 61100 + 2 * 62400, SDS-PAGE Bos taurus
62400
-
 2 * 61100 + 2 * 62400, SDS-PAGE Bos taurus
63300
-
 4 * 63300, SDS-PAGE Rattus norvegicus
210000 211000 gel filtration Bos taurus
239000
-
 brain, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2 Rattus norvegicus rate-limiting step in catecholamine biosynthesis 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
L-tyrosine + tetrahydrobiopterin + O2 Bos taurus rate-limiting step in catecholamine biosynthesis 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 kinetically distinguishable forms of the enzyme: low Km form and high Km form Rattus norvegicus
from adrenal medulla Bos taurus
from brain Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
 Rattus norvegicus
-
adrenal medulla
-
 Bos taurus
-
brain
-
 Rattus norvegicus
-
brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0916
-
 purified enzyme from brain Rattus norvegicus
0.18
-
 purified enzyme from adrenal medulla Bos taurus

Storage Stability

Storage Stability Organism
-70°C, in presence of diisopropylfluorophosphate, 2 months Bos taurus
4°C, in presence of diisopropylfluorophosphate, 1 week Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
L-tyrosine + 6-methyltetrahydropterin + O2
-
 Bos taurus ?
-
 ?
L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
L-tyrosine + tetrahydrobiopterin + O2
-
 Bos taurus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
L-tyrosine + tetrahydrobiopterin + O2 rate-limiting step in catecholamine biosynthesis Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
L-tyrosine + tetrahydrobiopterin + O2 rate-limiting step in catecholamine biosynthesis Bos taurus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 63300, SDS-PAGE Rattus norvegicus
tetramer 2 * 61100 + 2 * 62400, SDS-PAGE Bos taurus