Literature summary for 1.14.16.1 extracted from

Carluccio, C.; Fraternali, F.; Salvatore, F.; Fornili, A.; Zagari, A.
Towards the identification of the allosteric Phe-binding site in phenylalanine hydroxylase (2016), J. Biomol. Struct. Dyn., 34, 497-507 .

Search for more literature for 1.14.16.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BW25113DELTAtnaA cells	Pseudomonas fluorescens
expressed in Escherichia coli BW25113DELTAtnaA cells	Pseudomonas aeruginosa
expressed in Escherichia coli BW25113DELTAtnaA cells	Pseudomonas putida
expressed in Escherichia coli BW25113DELTAtnaA cells	Cupriavidus necator
expressed in Escherichia coli BW25113DELTAtnaA cells	Xanthomonas campestris

Protein Variants

Protein Variants	Comment	Organism
G46S	the mutant shows lower allosteric Phe-binding ability compared to the wild type enzyme	Homo sapiens
W179/L98Y	the mutant shows 46% activity with L-phenylalanine and 1207% activity with L-tryptophan compared to the wild type enzyme	Xanthomonas campestris
W179F	the mutant shows 80% activity with L-phenylalanine and 1739% activity with L-tryptophan compared to the wild type enzyme	Xanthomonas campestris
W179F/L98Y/Y231C	the mutant shows 17% activity with L-phenylalanine and 570% activity with L-tryptophan compared to the wild type enzyme	Xanthomonas campestris
W179F/Y231C	the mutant shows 52% activity with L-phenylalanine and 952% activity with L-tryptophan compared to the wild type enzyme	Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
L-phenylalanine + tetrahydrobiopterin + O2	Pseudomonas fluorescens	-	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	Pseudomonas aeruginosa	-	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	Pseudomonas putida	-	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	Cupriavidus necator	-	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	Xanthomonas campestris	-	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	Homo sapiens	-	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	-	-	-
Homo sapiens	P00439	-	-
Pseudomonas aeruginosa	-	-	-
Pseudomonas fluorescens	-	-	-
Pseudomonas putida	-	-	-
Xanthomonas campestris	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-phenylalanine + tetrahydrobiopterin + O2	-	Pseudomonas fluorescens	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	-	Pseudomonas aeruginosa	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	-	Pseudomonas putida	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	-	Cupriavidus necator	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	-	Xanthomonas campestris	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	-	Homo sapiens	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	preferred substrate	Pseudomonas fluorescens	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	preferred substrate	Pseudomonas aeruginosa	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	preferred substrate	Pseudomonas putida	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	preferred substrate	Cupriavidus necator	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + tetrahydrobiopterin + O2	preferred substrate	Xanthomonas campestris	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-tryptophan + tetrahydrobiopterin + O2	-	Pseudomonas fluorescens	5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin	-	?
L-tryptophan + tetrahydrobiopterin + O2	-	Pseudomonas aeruginosa	5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin	-	?
L-tryptophan + tetrahydrobiopterin + O2	-	Pseudomonas putida	5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin	-	?
L-tryptophan + tetrahydrobiopterin + O2	-	Cupriavidus necator	5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin	-	?
L-tryptophan + tetrahydrobiopterin + O2	-	Xanthomonas campestris	5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
P4H	-	Pseudomonas fluorescens
P4H	-	Pseudomonas aeruginosa
P4H	-	Pseudomonas putida
P4H	-	Cupriavidus necator
P4H	-	Xanthomonas campestris
PAH	-	Homo sapiens
phenylalanine 4-hydroxylase	-	Pseudomonas fluorescens
phenylalanine 4-hydroxylase	-	Pseudomonas aeruginosa
phenylalanine 4-hydroxylase	-	Pseudomonas putida
phenylalanine 4-hydroxylase	-	Cupriavidus necator
phenylalanine 4-hydroxylase	-	Xanthomonas campestris
phenylalanine hydroxylase	-	Pseudomonas fluorescens
phenylalanine hydroxylase	-	Pseudomonas aeruginosa
phenylalanine hydroxylase	-	Pseudomonas putida
phenylalanine hydroxylase	-	Cupriavidus necator
phenylalanine hydroxylase	-	Xanthomonas campestris
phenylalanine hydroxylase	-	Homo sapiens
phhA	-	Pseudomonas fluorescens
phhA	-	Pseudomonas aeruginosa
phhA	-	Pseudomonas putida
phhA	-	Cupriavidus necator
phhA	-	Xanthomonas campestris