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Literature summary for 1.14.16.1 extracted from

  • Leandro, J.; Stokka, A.J.; Teigen, K.; Andersen, O.A.; Flatmark, T.
    Substituting Tyr138 in the active site loop of human phenylalanine hydroxylase affects catalysis and substrate activation (2017), FEBS Open Bio, 7, 1026-1036 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TB1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y138A the mutant shows reduced catalytic efficiency (about 38%) compared to the wild type enzyme Homo sapiens
Y138E the mutant shows reduced catalytic efficiency (about 15%) compared to the wild type enzyme Homo sapiens
Y138F the mutant shows reduced catalytic efficiency (about 55%) compared to the wild type enzyme Homo sapiens
Y138K the mutant shows severely reduced catalytic efficiency (about 5%) compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
tetrahydrobiopterin mutant enzyme Y138E, at pH 7.0 and 25°C Homo sapiens
0.035
-
tetrahydrobiopterin mutant enzyme Y138F, at pH 7.0 and 25°C Homo sapiens
0.039
-
tetrahydrobiopterin wild type enzyme, at pH 7.0 and 25°C Homo sapiens
0.044
-
tetrahydrobiopterin mutant enzyme Y138K, at pH 7.0 and 25°C Homo sapiens
0.047
-
tetrahydrobiopterin mutant enzyme Y138A, at pH 7.0 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 Homo sapiens
-
L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P00439
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
Homo sapiens L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 50000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
PAH
-
Homo sapiens
phenylalanine hydroxylase
-
Homo sapiens