Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.1 extracted from

  • Ye, S.; Zhou, L.; Zhou, Z.
    Thermal stability improvement for phenylalanine hydroxylase by site-directed mutagenesis (2016), Chin. J. Biotechnol., 32, 1243-1254 .
    View publication on PubMed
Search for more literature for 1.14.16.1

Protein Variants

Protein Variants Comment Organism
G221A the half-life of the mutant at 50°C is 16.8 min, which is increased by 0.9-times compared to the wild type enzyme Chromobacterium violaceum
K94R the half-life of the mutant at 50°C is 26.2 min, which is increased by 1.9-times compared to the wild type enzyme Chromobacterium violaceum
K94R/G221A the residual activity of the mutant is improved to 65.6% after keeping at 50°C for 1 h, which is 6.6 time higher than the wild type enzyme Chromobacterium violaceum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 Chromobacterium violaceum
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum P30967
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
 Chromobacterium violaceum L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?

Synonyms

Synonyms Comment Organism
PAH
-
 Chromobacterium violaceum
phenylalanine hydroxylase
-
 Chromobacterium violaceum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the residual activity of the enzyme is 8.6% and the half-life is 9 min when incubated at 50°C for 1 h Chromobacterium violaceum