Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-phenylalanine | preincubation with L-phenylalanine exhibits 1.3fold stimulatory effect on kcat | Dictyostelium discoideum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)/pLysS cells | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.287 | - |
L-phenylalanine | wild type enzyme, 5 min preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C | Dictyostelium discoideum | |
0.637 | - |
L-phenylalanine | wild type enzyme, without preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C | Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | Dictyostelium discoideum | - |
L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | Dictyostelium discoideum AX2 | - |
L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Dictyostelium discoideum AX2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Dictyostelium discoideum | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Dictyostelium discoideum AX2 | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Dictyostelium discoideum |
Synonyms | Comment | Organism |
---|---|---|
PAH | - |
Dictyostelium discoideum |
phenylalanine hydroxylase | - |
Dictyostelium discoideum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.62 | - |
L-phenylalanine | wild type enzyme, without preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C | Dictyostelium discoideum | |
8.93 | - |
L-phenylalanine | wild type enzyme, 5 min preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C | Dictyostelium discoideum |