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Literature summary for 1.14.16.1 extracted from

  • Kim, H.L.; Park, M.B.; Kim, Y.; Yang, Y.G.; Lee, S.W.; Zhuang, N.; Lee, K.H.; Park, Y.S.
    Dictyostelium phenylalanine hydroxylase is activated by its substrate phenylalanine (2012), FEBS Lett., 586, 3596-3600.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-phenylalanine preincubation with L-phenylalanine exhibits 1.3fold stimulatory effect on kcat Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)/pLysS cells Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.287
-
L-phenylalanine wild type enzyme, 5 min preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C Dictyostelium discoideum
0.637
-
L-phenylalanine wild type enzyme, without preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 Dictyostelium discoideum
-
L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?
L-phenylalanine + tetrahydrobiopterin + O2 Dictyostelium discoideum AX2
-
L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Dictyostelium discoideum AX2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
Dictyostelium discoideum L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?
L-phenylalanine + tetrahydrobiopterin + O2
-
Dictyostelium discoideum AX2 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?

Subunits

Subunits Comment Organism
tetramer
-
Dictyostelium discoideum

Synonyms

Synonyms Comment Organism
PAH
-
Dictyostelium discoideum
phenylalanine hydroxylase
-
Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.62
-
L-phenylalanine wild type enzyme, without preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C Dictyostelium discoideum
8.93
-
L-phenylalanine wild type enzyme, 5 min preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C Dictyostelium discoideum