Protein Variants | Comment | Organism |
---|---|---|
Y325A | high degree of aggregation, but suffivcient tetrameric form for characterization | Homo sapiens |
Y325F | stable, similar yields and oligomeric distribution as wild-type, posttranslational hydroxylation | Homo sapiens |
Y325L | stable, similar yields and oligomeric distribution as wild-type, reduced specific activity, decreased coupling efficiency and decreased iron content, no positive cooperativity for L-phenylalanine | Homo sapiens |
Y325S | aggregation after purification, not suitable for characterization | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
Abz-VAA | mutant Y325L, 25°C, pH 7.0 | Homo sapiens | |
0.008 | - |
Abz-VAA | mutant enzyme Y325L, 25°C, pH 7.0 | Homo sapiens | |
0.027 | - |
Abz-VAA | mutant Y325F, 25°C, pH 7.0 | Homo sapiens | |
0.027 | - |
Abz-VAA | mutant enzyme Y325F, 25°C, pH 7.0 | Homo sapiens | |
0.028 | - |
Abz-VAA | wild-type, 25°C, pH 7.0 | Homo sapiens | |
0.028 | - |
Abz-VAA | wild type enzyme, 25°C, pH 7.0 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
290 | - |
mutant Y325A, 25°C, pH 7.0 | Homo sapiens |
300 | - |
mutant Y325A, preincubation with L-phenylalanine, 25°C, pH 7.0 | Homo sapiens |
1150 | - |
mutant Y325F, 25°C, pH 7.0 | Homo sapiens |
1230 | - |
wild-type, 25°C, pH 7.0 | Homo sapiens |
1310 | - |
mutant Y325L, 25°C, pH 7.0 | Homo sapiens |
1500 | - |
mutant Y325L, preincubation with L-phenylalanine, 25°C, pH 7.0 | Homo sapiens |
3630 | - |
mutant Y325F, preincubation with L-phenylalanine, 25°C, pH 7.0 | Homo sapiens |
3640 | - |
wild-type, preincubation with L-phenylalanine, 25°C, pH 7.0 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-VAA + tetrahydrobiopterin + O2 | - |
Homo sapiens | ? | - |
r | |
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Homo sapiens |