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Literature summary for 1.14.16.1 extracted from

  • Leandro, P.; Lechner, M.C.; Tavares de Almeida, I.; Konecki, D.
    Glycerol increases the yield and activity of human phenylalanine hydroxylase mutant enzymes produced in a prokaryotic expression system (2001), Mol. Genet. Metab., 73, 173-178.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, R270K and V388M mutant enzymes in the presence of the chemical chaperone glycerol Homo sapiens

Protein Variants

Protein Variants Comment Organism
R270K expression in the presence of the chemical chaperone glycerol enhances activity after purification Homo sapiens
V388M expression in the presence of the chemical chaperone glycerol enhances activity after purification Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00439
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type, R270K and V388M mutant enzymes expressed in the presence and absence of glycerol Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.099
-
R270K mutant enzyme, expression in the absence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin Homo sapiens
0.23
-
R270K mutant enzyme, expression in the presence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin Homo sapiens
1.773
-
V388M mutant enzyme, expression in the absence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin Homo sapiens
2.91
-
V388M mutant enzyme, expression in the presence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin Homo sapiens