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Literature summary for 1.14.16.1 extracted from

  • Martinez, A.; Knappskog, P.M.; Olafsdottir, S.; Doskeland, A.P.; Eiken, H.G.; Svebak, R.M.; Bozzini, M.; Apold, J.; Flatmark, T.
    Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme (1995), Biochem. J., 306, 589-597.
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Cloned(Commentary)

Cloned (Comment) Organism
expression as maltose-binding-protein fusion protein in Escherichia coli circumvents proteolytic degradation by the host cell Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 tetrahydrobiopterin cleaved maltose-binding-protein phenylalanine fusion protein Homo sapiens
0.029
-
 tetrahydrobiopterin recombinant enzyme Homo sapiens
0.031
-
 tetrahydrobiopterin maltose-binding-protein phenylalanine fusion protein Homo sapiens
0.073
-
 6-methyltetrahydropterin maltose-binding-protein phenylalanine fusion protein Homo sapiens
0.088
-
 6-methyltetrahydropterin recombinant enzyme Homo sapiens
0.175
-
 L-phenylalanine cofactor tetrahydrobiopterin, recombinant enzyme Homo sapiens
0.194
-
 L-phenylalanine cofactor tetrahydrobiopterin, maltose-binding-protein phenylalanine fusion protein Homo sapiens
0.236
-
 L-phenylalanine cofactor tetrahydrobiopterin, cleaved maltose-binding-protein phenylalanine fusion protein Homo sapiens
0.382
-
 L-phenylalanine cofactor 6-methyltetrahydropterin, recombinant enzyme Homo sapiens
0.504
-
 L-phenylalanine cofactor 6-methyltetrahydropterin, maltose-binding-protein phenylalanine fusion protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding-protein phenylalanine hydroxylase fusion protein Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.424
-
 maltose-binding-protein phenylalanine hydroxylase fusion protein, dimeric form Homo sapiens
1.283
-
 maltose-binding-protein phenylalanine hydroxylase fusion protein, tetrameric form Homo sapiens