Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Chlorophenylalanine | recombinant enzyme, 3 mM, almost complete inhibition | Homo sapiens | |
tryptophan | recombinant enzyme, 3 mM, approx. 75% inhibition | Homo sapiens | |
tyrosine | recombinant enzyme, 3 mM, approx. 50% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 0.015 | 6-methyltetrahydropterin | - |
Homo sapiens | |
0.016 | - |
tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
0.023 | - |
tetrahydrobiopterin | native liver enzyme in crude extract | Homo sapiens | |
0.05 | 0.075 | L-phenylalanine | recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
0.09 | - |
L-phenylalanine | native liver enzyme in crude extract, cofactor 6-methyltetrahydropterin | Homo sapiens | |
0.3 | 0.4 | L-phenylalanine | native and recombinant enzyme, cofactor 6-methyltetrahydropterin | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, partially purified | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
native and recombinant enzyme | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 42 | native and recombinant enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Homo sapiens |