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Literature summary for 1.14.16.1 extracted from
- Biochemical characterization of recombinant human phenylalanine hydroxylase produced in Escherichia coli (1987), J. Biol. Chem., 262, 2228-2233.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Chlorophenylalanine | recombinant enzyme, 3 mM, almost complete inhibition | Homo sapiens |  |
| tryptophan | recombinant enzyme, 3 mM, approx. 75% inhibition | Homo sapiens | |
| tyrosine | recombinant enzyme, 3 mM, approx. 50% inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | 0.015 | 6-methyltetrahydropterin | - |
Homo sapiens | |
| 0.016 | - |
tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
| 0.023 | - |
tetrahydrobiopterin | native liver enzyme in crude extract | Homo sapiens | |
| 0.05 | 0.075 | L-phenylalanine | recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| 0.09 | - |
L-phenylalanine | native liver enzyme in crude extract, cofactor 6-methyltetrahydropterin | Homo sapiens | |
| 0.3 | 0.4 | L-phenylalanine | native and recombinant enzyme, cofactor 6-methyltetrahydropterin | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, partially purified | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
native and recombinant enzyme | Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 42 | native and recombinant enzyme | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrobiopterin | - |
Homo sapiens | |
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