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Literature summary for 1.14.15.8 extracted from
- Engineering of CYP106A2 for steroid 9alpha- and 6beta-hydroxylation (2017), Steroids, 120, 41-48 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A243S | mutant engineered for steroid 9alpha- and 6beta-hydroxylation, 10fold increase in the selectivity towards 6alpha-hydroxylation of progesterone. Mutant produces 3.4% 15beta-hydroxy-progesterone plus 4.6% 11alpha-hydroxy-progesterone plus 2.4% 9alpha-hydroxy-progesterone plus 82.7% 6beta-hydroxy-progesterone | Priestia megaterium |
| F165L/A395E/G397V | mutant engineered for steroid 9alpha- and 6beta-hydroxylation, 10fold increase in the selectivity towards progesterone 9lpha-hydroxylation. Mutant produces 11.3% 15beta-hydroxy-progesterone plus 17.2% 11alpha-hydroxy-progesterone plus 59.7% 9alpha-hydroxy-progesterone plus 6.8% 6beta-hydroxy-progesterone | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | Q06069 | isoform Cyp106A2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| progesterone + reduced adrenodoxin + O2 | - |
Priestia megaterium | 15beta-hydroxy-progesterone + 11alpha-hydroxy-progesterone + oxidized adrenodoxin + H2O | - |
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