BRENDA - Enzyme Database
show all sequences of 1.14.15.6

Import of hybrid forms of CYP11A1 into yeast mitochondria

Minenko, A.N.; Novikova, L.A.; Luzikov, V.N.; Kovaleva, I.E.; Biochim. Biophys. Acta 1780, 1121-1130 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
hybrid proteins AAC-mCYP11A1, Bcs1p(183)-mCYP11A1, Su9(1-116)-mCYP11A1, preAd-mCYP11A1, DLD(1-72)-mCYP11A1 and COXIV-mCYP11A1 expressed in Saccharomyces cerevisiae strain 2805. Escherichia coli strain JM109 transformed with the plasmid pTrc99(A)/Ad-mCYP11A1
synthetic construct
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
all of the addressing sequences, containing transmembrane domains, provide effective insertion of the hybrid proteins AAC-mCYP11A1, Bcs1p(1-83)-mCYP11A1, DLD(1-72)-mCYP11A1 and Su9(1-116)-mCYP11A1 into the mitochondrial inner membrane. preAd-mCYP11A1 hybrid molecules are translocated across the inner membrane and tightly associated with the membrane on its matrix side but not membrane inserted. The mechanism of Ad-mCYP11A1 hybrid topogenesis in Escherichia coli cells differs from that of the topogenesis of its precursor form in yeast mitochondria
synthetic construct
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
synthetic construct
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
22(R)-hydroxycholesterol + reduced adrenodoxin + O2
mitochondria containing the AAC-mCYP11A1 hybrid have the highest cholesterol side-chain cleavage activity in the reconstituted system. Ad-mCYP11A1 hybrid demonstrates only residual enzyme activity
702403
synthetic construct
? + oxidized adrenodoxin + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
adrenodoxin
-
synthetic construct
Cloned(Commentary) (protein specific)
Commentary
Organism
hybrid proteins AAC-mCYP11A1, Bcs1p(183)-mCYP11A1, Su9(1-116)-mCYP11A1, preAd-mCYP11A1, DLD(1-72)-mCYP11A1 and COXIV-mCYP11A1 expressed in Saccharomyces cerevisiae strain 2805. Escherichia coli strain JM109 transformed with the plasmid pTrc99(A)/Ad-mCYP11A1
synthetic construct
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
adrenodoxin
-
synthetic construct
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
all of the addressing sequences, containing transmembrane domains, provide effective insertion of the hybrid proteins AAC-mCYP11A1, Bcs1p(1-83)-mCYP11A1, DLD(1-72)-mCYP11A1 and Su9(1-116)-mCYP11A1 into the mitochondrial inner membrane. preAd-mCYP11A1 hybrid molecules are translocated across the inner membrane and tightly associated with the membrane on its matrix side but not membrane inserted. The mechanism of Ad-mCYP11A1 hybrid topogenesis in Escherichia coli cells differs from that of the topogenesis of its precursor form in yeast mitochondria
synthetic construct
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
22(R)-hydroxycholesterol + reduced adrenodoxin + O2
mitochondria containing the AAC-mCYP11A1 hybrid have the highest cholesterol side-chain cleavage activity in the reconstituted system. Ad-mCYP11A1 hybrid demonstrates only residual enzyme activity
702403
synthetic construct
? + oxidized adrenodoxin + H2O
-
-
-
?
Other publictions for EC 1.14.15.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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30-36
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745750
Efimova
Expression of cholesterol hyd ...
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59
394-406
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744728
Kubeil
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Membrane-mediated protein-pro ...
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ChemPlusChem
81
995-1002
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744345
Davydov
Evidence that compound I is t ...
Homo sapiens
Biochemistry
54
7089-7097
2015
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745591
Cook
Overexpression of the steroid ...
Rattus norvegicus
J. Neurosci.
34
5824-5834
2014
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746528
Zhang
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The influence of the adjacent ...
synthetic construct
Theoret. Chem. Accounts
133
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2014
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Schiffler
Kinetic and optical biosensor ...
Homo sapiens
Eur. Biophys. J.
40
1275-1282
2011
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725459
Mast
Structural basis for three-ste ...
Bos taurus
J. Biol. Chem.
286
5607-5613
2011
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725962
Pagotto
Localization and functional ac ...
Rattus norvegicus, Rattus norvegicus Wistar
Mol. Cell. Endocrinol.
332
253-260
2011
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711826
Nakamoto
Cloning and expression of meda ...
Oryzias latipes
Dev. Growth Differ.
52
385-395
2010
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712096
Evans
Regulation of mRNAs encoding t ...
Dasyatis sabina
Gen. Comp. Endocrinol.
168
121-132
2010
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712924
Warita
Direct effects of diethylstilb ...
Mus musculus
Life Sci.
87
281-285
2010
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713379
Slominski
Products of vitamin D3 or 7-de ...
Homo sapiens
PLoS ONE
5
e9907
2010
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703418
Nguyen
Metabolism of vitamin d2 to 17 ...
Bos taurus
Drug Metab. Dispos.
37
761-767
2009
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4
1
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703876
Spivak
Construction and characteristi ...
Bos taurus
Genetika
45
1217-1224
2009
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705739
Teplyuk
The osteogenic transcription f ...
Homo sapiens, Mus musculus
Mol. Endocrinol.
23
849-861
2009
2
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2
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2
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706698
Irving-Rodgers
Studies of granulosa cell matu ...
Bos taurus
Reproduction
137
825-834
2009
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706699
Rangel
Testosterone stimulates proges ...
Gallus gallus
Reproduction
138
961-969
2009
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686218
Miura
Differentiation of ambisexual ...
Amphiprion clarkii
Comp. Biochem. Physiol. B
149
29-37
2008
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687970
Kim
Severe combined adrenal and go ...
Homo sapiens
J. Clin. Endocrinol. Metab.
93
696-702
2008
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687991
Martinez
Immunolocalization of choleste ...
Mytilus galloprovincialis
J. Comp. Physiol. B
178
647-654
2008
1
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688147
Chiu
Generation and utilization of ...
Bos taurus, Capra aegagrus, Sus scrofa
J. Immunoassay Immunochem.
29
152-160
2008
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3
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3
3
3
6
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4
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8
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3
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6
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3
6
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3
3
3
6
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8
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6
3
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702403
Minenko
Import of hybrid forms of CYP1 ...
synthetic construct
Biochim. Biophys. Acta
1780
1121-1130
2008
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1
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702781
Carrara
Screen-printed electrodes base ...
synthetic construct
Biosens. Bioelectron.
24
148-150
2008
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Steroidogenic acute regulatory ...
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703625
Tuckey
Pathways and products for the ...
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704039
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Kinetics of vitamin D3 metabol ...
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705461
Pikuleva
Studies of membrane topology o ...
Bos taurus
Lipids
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Cholesterol-metabolizing cytoc ...
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Drug Metab. Dispos.
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Changes in localization of cyt ...
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Leptin interferes with adrenoc ...
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Cloning and characterization o ...
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StAR and progesterone producin ...
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Modulation of brain steroidoge ...
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Rekawiecki
Stimulatory effect of LH, PGE2 ...
Bos taurus
Prostaglandins other Lipid Mediat.
78
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2005
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658508
Nazarov
Formation and functioning of f ...
Bos taurus, Homo sapiens
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22
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2
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Patte-Mensah
Cellular distribution and bioa ...
Rattus norvegicus
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86
1233-1246
2003
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Usanov
Probing the interaction of bov ...
Bos taurus
Biochemistry
41
8310-8320
2002
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657647
Headlam
The effect of glycerol on cyto ...
Bos taurus
Arch. Biochem. Biophys.
407
95-102
2002
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Vilchis
The cDNA cloning and tissue ex ...
Mesocricetus auratus
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659805
Tuckey
Placental cytochrome P450scc ( ...
Homo sapiens
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81
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2002
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660564
Endo
Localization of the cytochrome ...
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19
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2002
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285430
Lepesheva
Site-directed mutagenesis of c ...
Bos taurus
Biochemistry (Moscow)
65
1409-1418
2000
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1
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10
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285431
Ahmed
The use of the novel substrate ...
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Biochem. Biophys. Res. Commun.
274
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2000
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Schwarz
alpha-Branched 1,2-diacyl phos ...
Bos taurus
J. Biol. Chem.
271
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1996
4
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1
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2
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1
1
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285428
Miao
Cytochrome P-450scc-mediated o ...
Bos taurus
Biochemistry
34
8415-8421
1995
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1
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1
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1
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285432
Tsujita
Substrate-binding region of cy ...
Bos taurus
Biochim. Biophys. Acta
1161
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1993
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4
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3
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1
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285420
Dhariwal
Cholesterol metabolism by puri ...
Bos taurus
Biochemistry
28
8397-8402
1989
4
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2
3
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1
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285421
Lambeth
Cytochrome P-450scc-adrenodoxi ...
Bos taurus
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260
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1985
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285422
Nagahisa
Competitive inhibition of cyto ...
Bos taurus
J. Biol. Chem.
260
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1985
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2
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2
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285417
Hume
The catalytic cycle of cytochr ...
Bos taurus
Eur. J. Biochem.
140
583-591
1984
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1
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285424
Sheets
Active site-directed inhibitor ...
Bos taurus
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258
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1983
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8
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285425
Sheets
C-22-Substituted steroid deriv ...
Bos taurus
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258
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1983
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285426
Warne
Modulation of the kinetics of ...
Bos taurus
Proc. Natl. Acad. Sci. USA
80
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1983
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1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285413
Hanukoglu
Adrenal mitochondrial cytochro ...
Bos taurus
J. Biol. Chem.
256
4321-4328
1981
2
-
-
-
-
-
1
2
1
3
-
-
-
1
-
-
1
-
-
1
-
3
3
-
1
-
1
-
-
-
-
3
-
-
-
2
-
-
3
-
-
-
-
1
-
2
1
3
-
-
-
-
-
1
-
1
-
3
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285414
Hanukoglu
Mechanisms of ionic activation ...
Bos taurus
J. Biol. Chem.
256
4329-4335
1981
1
-
-
-
-
-
2
-
1
3
-
-
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
2
-
-
1
3
-
-
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285412
Hanukoglu
Mitochondrial cytochrome P-450 ...
Bos taurus
J. Biol. Chem.
255
3057-3061
1980
-
-
-
-
-
-
1
2
1
-
-
1
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
2
1
-
-
1
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285423
Kashiwagi
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
255
2606-2611
1980
-
-
-
-
-
-
-
-
2
-
12
1
-
2
-
-
1
-
-
4
-
1
2
1
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
2
-
12
1
-
-
-
1
-
4
-
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285416
Nakajin
Binding of Triton X-100 to pur ...
Bos taurus
Biochem. Biophys. Res. Commun.
87
524-531
1979
4
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
1
2
-
-
-
-
-
1
-
-
2
-
-
-
4
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285415
Wang
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
251
6068-6074
1976
-
-
-
-
-
-
2
-
1
-
3
1
-
3
-
-
1
-
-
1
-
3
2
1
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
2
-
-
1
-
3
1
-
-
-
1
-
1
-
3
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285419
Takemori
Purification of cytochrome P-4 ...
Bos taurus
Biochem. Biophys. Res. Commun.
63
588-593
1975
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
1
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285418
Shikita
Cytochrome P-450 from bovine a ...
Bos taurus
J. Biol. Chem.
248
5598-5604
1973
2
-
-
-
-
1
-
2
1
-
2
1
-
1
-
-
1
-
-
1
-
2
3
-
1
-
-
-
2
1
-
3
-
-
-
2
-
-
3
-
-
1
-
-
-
2
1
-
2
1
-
-
-
1
-
1
-
2
3
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
285427
Shikita
Cytochrome P-450 from bovine a ...
Bos taurus
J. Biol. Chem.
248
5605-5609
1973
1
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
3
-
-
-
1
-
-
3
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-