BRENDA - Enzyme Database
show all sequences of 1.14.15.6

Purification and characterization of mitochondrial cytochrome P-450 associated with cholesterol side chain cleavage from bovine corpus luteum

Kashiwagi, K.; Dafeldecker, W.P.; Salhanick, H.A.; J. Biol. Chem. 255, 2606-2611 (1980)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
-
Bos taurus
5743
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
48000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
53000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
60000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
97000
-
sedimentation equilibrium
Bos taurus
100000
-
native-PAGE, incomplete enzyme or molecular environmental conditions not optimal
Bos taurus
200000
-
native-PAGE, lowest single unit with high enzymatic activity
Bos taurus
220000
-
corpus luteum
Bos taurus
225000
-
sedimentation equilibrium
Bos taurus
400000
-
native-PAGE
Bos taurus
415000
-
sedimentation equilibrium
Bos taurus
850000
-
adrenal mitochondria
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + 6 reduced adrenodoxin + 3 O2
Bos taurus
first step in biosynthesis of all steroid hormones
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
Bos taurus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
several ion exchange and heptyl-Sepharose hydrophobic chromatography
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Bos taurus
-
corpus luteum
-
Bos taurus
-
Storage Stability
Storage Stability
Organism
0C on ice, purified, 20 mM potassium phosphate buffer, pH 7.4, 20% glycerol, v/v, 0.1 mM EDTA, 0.1 mM dithioerythritol, 0.01% Emulgen 911, 3 weeks, without substantial loss of activity
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + 6 reduced adrenodoxin + 3 O2
first step in biosynthesis of all steroid hormones
285423
Bos taurus
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
285423
Bos taurus
?
cholesterol + reduced adrenodoxin + O2
-
285423
Bos taurus
pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
285423
Bos taurus
?
Subunits
Subunits
Commentary
Organism
?
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
adrenodoxin
-
Bos taurus
heme
0.78 heme group/unit of 48000 Da P-450, 1 mol of iron/44000 g
Bos taurus
NADPH
NADPH-linked adrenodoxin reductase
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
adrenodoxin
-
Bos taurus
heme
0.78 heme group/unit of 48000 Da P-450, 1 mol of iron/44000 g
Bos taurus
NADPH
NADPH-linked adrenodoxin reductase
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
-
Bos taurus
5743
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
48000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
53000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
60000
-
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
97000
-
sedimentation equilibrium
Bos taurus
100000
-
native-PAGE, incomplete enzyme or molecular environmental conditions not optimal
Bos taurus
200000
-
native-PAGE, lowest single unit with high enzymatic activity
Bos taurus
220000
-
corpus luteum
Bos taurus
225000
-
sedimentation equilibrium
Bos taurus
400000
-
native-PAGE
Bos taurus
415000
-
sedimentation equilibrium
Bos taurus
850000
-
adrenal mitochondria
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + 6 reduced adrenodoxin + 3 O2
Bos taurus
first step in biosynthesis of all steroid hormones
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
Bos taurus
?
Purification (Commentary) (protein specific)
Commentary
Organism
several ion exchange and heptyl-Sepharose hydrophobic chromatography
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Bos taurus
-
corpus luteum
-
Bos taurus
-
Storage Stability (protein specific)
Storage Stability
Organism
0C on ice, purified, 20 mM potassium phosphate buffer, pH 7.4, 20% glycerol, v/v, 0.1 mM EDTA, 0.1 mM dithioerythritol, 0.01% Emulgen 911, 3 weeks, without substantial loss of activity
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + 6 reduced adrenodoxin + 3 O2
first step in biosynthesis of all steroid hormones
285423
Bos taurus
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
285423
Bos taurus
?
cholesterol + reduced adrenodoxin + O2
-
285423
Bos taurus
pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
285423
Bos taurus
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria
Bos taurus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Bos taurus
Other publictions for EC 1.14.15.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743937
Wang
-
Effect of heshouwuyin on prot ...
Rattus norvegicus
Acta Anatomica Sinica
48
30-36
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
745750
Efimova
Expression of cholesterol hyd ...
Bos taurus
Mol. Biotechnol.
59
394-406
2017
-
1
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744728
Kubeil
-
Membrane-mediated protein-pro ...
Bos taurus
ChemPlusChem
81
995-1002
2016
-
1
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
744345
Davydov
Evidence that compound I is t ...
Homo sapiens
Biochemistry
54
7089-7097
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745591
Cook
Overexpression of the steroid ...
Rattus norvegicus
J. Neurosci.
34
5824-5834
2014
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746528
Zhang
-
The influence of the adjacent ...
synthetic construct
Theoret. Chem. Accounts
133
1-8
2014
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724894
Schiffler
Kinetic and optical biosensor ...
Homo sapiens
Eur. Biophys. J.
40
1275-1282
2011
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725459
Mast
Structural basis for three-ste ...
Bos taurus
J. Biol. Chem.
286
5607-5613
2011
-
-
1
1
-
-
-
-
1
-
-
4
-
1
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
1
-
-
-
-
-
-
1
-
-
4
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725962
Pagotto
Localization and functional ac ...
Rattus norvegicus, Rattus norvegicus Wistar
Mol. Cell. Endocrinol.
332
253-260
2011
-
-
-
-
-
-
-
-
1
-
-
8
-
167
-
-
1
-
-
5
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
8
-
-
-
1
-
5
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711826
Nakamoto
Cloning and expression of meda ...
Oryzias latipes
Dev. Growth Differ.
52
385-395
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712096
Evans
Regulation of mRNAs encoding t ...
Dasyatis sabina
Gen. Comp. Endocrinol.
168
121-132
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712924
Warita
Direct effects of diethylstilb ...
Mus musculus
Life Sci.
87
281-285
2010
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
713379
Slominski
Products of vitamin D3 or 7-de ...
Homo sapiens
PLoS ONE
5
e9907
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
703418
Nguyen
Metabolism of vitamin d2 to 17 ...
Bos taurus
Drug Metab. Dispos.
37
761-767
2009
-
-
-
-
-
-
1
4
1
-
-
-
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
4
1
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
703876
Spivak
Construction and characteristi ...
Bos taurus
Genetika
45
1217-1224
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
705739
Teplyuk
The osteogenic transcription f ...
Homo sapiens, Mus musculus
Mol. Endocrinol.
23
849-861
2009
2
-
2
-
-
-
-
-
3
-
4
-
-
3
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
3
-
4
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
2
3
3
2
-
-
706698
Irving-Rodgers
Studies of granulosa cell matu ...
Bos taurus
Reproduction
137
825-834
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
706699
Rangel
Testosterone stimulates proges ...
Gallus gallus
Reproduction
138
961-969
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
686218
Miura
Differentiation of ambisexual ...
Amphiprion clarkii
Comp. Biochem. Physiol. B
149
29-37
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687970
Kim
Severe combined adrenal and go ...
Homo sapiens
J. Clin. Endocrinol. Metab.
93
696-702
2008
-
-
1
-
4
-
-
-
1
1
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
4
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687991
Martinez
Immunolocalization of choleste ...
Mytilus galloprovincialis
J. Comp. Physiol. B
178
647-654
2008
1
-
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688147
Chiu
Generation and utilization of ...
Bos taurus, Capra aegagrus, Sus scrofa
J. Immunoassay Immunochem.
29
152-160
2008
-
-
3
-
-
-
-
-
3
3
3
6
-
4
-
-
3
-
-
8
-
-
6
3
-
-
-
-
-
-
-
6
-
-
-
-
-
3
6
-
-
-
-
-
-
-
3
3
3
6
-
-
-
3
-
8
-
-
6
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702403
Minenko
Import of hybrid forms of CYP1 ...
synthetic construct
Biochim. Biophys. Acta
1780
1121-1130
2008
-
-
1
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702781
Carrara
Screen-printed electrodes base ...
synthetic construct
Biosens. Bioelectron.
24
148-150
2008
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703100
Arukwe
Steroidogenic acute regulatory ...
Gadus morhua, Oncorhynchus mykiss, Salmo salar
Cell Biol. Toxicol.
24
527-540
2008
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
-
-
-
3
3
3
3
-
-
703625
Tuckey
Pathways and products for the ...
Bos taurus
FEBS J.
275
2585-2596
2008
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704039
Tuckey
Kinetics of vitamin D3 metabol ...
Bos taurus
Int. J. Biochem. Cell Biol.
40
2619-2626
2008
1
-
-
-
-
-
-
5
2
-
-
-
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
5
2
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
705461
Pikuleva
Studies of membrane topology o ...
Bos taurus
Lipids
43
1127-1132
2008
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
673298
Pikuleva
Cholesterol-metabolizing cytoc ...
Homo sapiens
Drug Metab. Dispos.
34
513-520
2006
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
6
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
6
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673821
Ijiri
Changes in localization of cyt ...
Anguilla japonica
Gen. Comp. Endocrinol.
145
75-83
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
7
-
-
1
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675059
Hsu
-
Leptin interferes with adrenoc ...
Homo sapiens
J. Clin. Endocrinol. Metab.
91
:2761-2769
2006
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675689
Kazeto
Cloning and characterization o ...
Anguilla japonica
J. Steroid Biochem. Mol. Biol.
99
121-128
2006
1
-
1
-
-
-
-
-
-
1
-
-
-
4
-
-
1
-
-
4
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658388
Abd-Elaziz
StAR and progesterone producin ...
Homo sapiens
Cancer Sci.
96
232-239
2005
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673372
Arukwe
Modulation of brain steroidoge ...
Salmo salar
Environ. Sci. Technol.
39
9791-9798
2005
1
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676926
Rekawiecki
Stimulatory effect of LH, PGE2 ...
Bos taurus
Prostaglandins other Lipid Mediat.
78
169-184
2005
1
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658508
Nazarov
Formation and functioning of f ...
Bos taurus, Homo sapiens
DNA Cell Biol.
22
243-252
2003
-
-
-
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659756
Patte-Mensah
Cellular distribution and bioa ...
Rattus norvegicus
J. Neurochem.
86
1233-1246
2003
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285433
Usanov
Probing the interaction of bov ...
Bos taurus
Biochemistry
41
8310-8320
2002
-
-
1
-
16
-
-
-
1
-
-
1
-
3
-
-
1
-
-
1
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
16
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
657647
Headlam
The effect of glycerol on cyto ...
Bos taurus
Arch. Biochem. Biophys.
407
95-102
2002
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658811
Vilchis
The cDNA cloning and tissue ex ...
Mesocricetus auratus
Gen. Comp. Endocrinol.
126
279-286
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659805
Tuckey
Placental cytochrome P450scc ( ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
81
153-158
2002
-
-
-
-
-
-
-
1
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660564
Endo
Localization of the cytochrome ...
Heterocephalus glaber
Zool. Sci.
19
673-678
2002
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285430
Lepesheva
Site-directed mutagenesis of c ...
Bos taurus
Biochemistry (Moscow)
65
1409-1418
2000
-
-
1
-
10
-
-
-
1
-
-
1
-
2
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
10
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285431
Ahmed
The use of the novel substrate ...
Bos taurus
Biochem. Biophys. Res. Commun.
274
821-824
2000
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
285429
Schwarz
alpha-Branched 1,2-diacyl phos ...
Bos taurus
J. Biol. Chem.
271
12840-12846
1996
4
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
1
-
-
2
-
1
1
-
-
-
-
-
3
-
-
-
4
-
-
3
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
285428
Miao
Cytochrome P-450scc-mediated o ...
Bos taurus
Biochemistry
34
8415-8421
1995
-
1
-
-
-
-
4
-
1
-
-
1
-
2
-
-
1
-
-
2
-
-
4
-
1
-
-
1
1
-
-
3
-
-
-
-
1
-
3
-
-
-
-
4
-
-
1
-
-
1
-
-
-
1
-
2
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
285432
Tsujita
Substrate-binding region of cy ...
Bos taurus
Biochim. Biophys. Acta
1161
124-130
1993
-
-
-
-
4
-
2
1
2
-
-
1
-
2
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
4
-
-
2
-
1
2
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285420
Dhariwal
Cholesterol metabolism by puri ...
Bos taurus
Biochemistry
28
8397-8402
1989
4
-
-
-
-
-
-
2
3
-
-
1
-
3
-
-
1
-
-
2
-
-
3
-
1
-
-
2
1
-
-
2
-
-
-
4
-
-
2
-
-
-
-
-
-
2
3
-
-
1
-
-
-
1
-
2
-
-
3
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
285421
Lambeth
Cytochrome P-450scc-adrenodoxi ...
Bos taurus
J. Biol. Chem.
260
8810-8816
1985
-
-
-
-
-
-
-
-
1
1
-
1
-
2
-
-
1
-
-
1
-
-
6
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
1
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285422
Nagahisa
Competitive inhibition of cyto ...
Bos taurus
J. Biol. Chem.
260
846-851
1985
-
-
-
-
-
1
2
-
1
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
1
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
285417
Hume
The catalytic cycle of cytochr ...
Bos taurus
Eur. J. Biochem.
140
583-591
1984
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
1
-
-
1
-
-
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285424
Sheets
Active site-directed inhibitor ...
Bos taurus
J. Biol. Chem.
258
11446-11452
1983
-
-
-
-
-
-
8
-
1
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
-
1
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285425
Sheets
C-22-Substituted steroid deriv ...
Bos taurus
J. Biol. Chem.
258
1720-1725
1983
-
-
-
-
-
-
4
-
1
-
-
1
-
2
-
-
1
-
-
1
-
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
4
-
-
1
-
-
1
-
-
-
1
-
1
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285426
Warne
Modulation of the kinetics of ...
Bos taurus
Proc. Natl. Acad. Sci. USA
80
1877-1881
1983
1
-
-
-
-
-
3
4
2
-
-
1
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
3
-
4
2
-
-
1
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285413
Hanukoglu
Adrenal mitochondrial cytochro ...
Bos taurus
J. Biol. Chem.
256
4321-4328
1981
2
-
-
-
-
-
1
2
1
3
-
-
-
1
-
-
1
-
-
1
-
3
3
-
1
-
1
-
-
-
-
3
-
-
-
2
-
-
3
-
-
-
-
1
-
2
1
3
-
-
-
-
-
1
-
1
-
3
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285414
Hanukoglu
Mechanisms of ionic activation ...
Bos taurus
J. Biol. Chem.
256
4329-4335
1981
1
-
-
-
-
-
2
-
1
3
-
-
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
2
-
-
1
3
-
-
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285412
Hanukoglu
Mitochondrial cytochrome P-450 ...
Bos taurus
J. Biol. Chem.
255
3057-3061
1980
-
-
-
-
-
-
1
2
1
-
-
1
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
2
1
-
-
1
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285423
Kashiwagi
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
255
2606-2611
1980
-
-
-
-
-
-
-
-
2
-
12
1
-
2
-
-
1
-
-
4
-
1
2
1
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
2
-
12
1
-
-
-
1
-
4
-
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285416
Nakajin
Binding of Triton X-100 to pur ...
Bos taurus
Biochem. Biophys. Res. Commun.
87
524-531
1979
4
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
1
2
-
-
-
-
-
1
-
-
2
-
-
-
4
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285415
Wang
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
251
6068-6074
1976
-
-
-
-
-
-
2
-
1
-
3
1
-
3
-
-
1
-
-
1
-
3
2
1
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
2
-
-
1
-
3
1
-
-
-
1
-
1
-
3
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285419
Takemori
Purification of cytochrome P-4 ...
Bos taurus
Biochem. Biophys. Res. Commun.
63
588-593
1975
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
1
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285418
Shikita
Cytochrome P-450 from bovine a ...
Bos taurus
J. Biol. Chem.
248
5598-5604
1973
2
-
-
-
-
1
-
2
1
-
2
1
-
1
-
-
1
-
-
1
-
2
3
-
1
-
-
-
2
1
-
3
-
-
-
2
-
-
3
-
-
1
-
-
-
2
1
-
2
1
-
-
-
1
-
1
-
2
3
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
285427
Shikita
Cytochrome P-450 from bovine a ...
Bos taurus
J. Biol. Chem.
248
5605-5609
1973
1
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
3
-
-
-
1
-
-
3
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-