General Stability | Organism |
---|---|
deoxycorticosterone stabilizes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
deoxycorticosterone | identical for both enzymes activities | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47500 | - |
4 * 47500, SDS-PAGE | Bos taurus |
47500 | - |
x * 47500, SDS-PAGE | Bos taurus |
185000 | - |
exclusion chromatography | Bos taurus |
1000000 | - |
glycerol density gradient | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | corticosterone generally believed to be preferred substrate for 18-hydroxylase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification of 11beta-hydroxylase with 18-hydroxylase, ammonium sulfate, DEAE-chromatography | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | both hydroxylation activities seem to occur in a single enzyme | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001061 | - |
deoxycorticosterone, 18-hydroxylation activity in | Bos taurus |
0.01193 | - |
deoxycorticosterone, 11beta-hydroxylation activity | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 1 week, without significant loss of activity | Bos taurus |
4°C, 1 week, without significant loss of activity | Bos taurus |
4°C, sonicated mitochondria, stable | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | addition of adrenodoxin and adrenodoxin reductase | Bos taurus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
corticosterone + reduced adrenal ferredoxin + O2 | addition of adrenodoxin and adrenodoxin reductase | Bos taurus | 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
additional information | corticosterone generally believed to be preferred substrate for 18-hydroxylase | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47500, SDS-PAGE | Bos taurus |
tetramer | 4 * 47500, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bos taurus |