BRENDA - Enzyme Database
show all sequences of 1.14.15.30

Deletion of the gene encoding the reductase component of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione

Yeh, C.H.; Kuo, Y.S.; Chang, C.M.; Liu, W.H.; Sheu, M.L.; Meng, M.; Microb. Cell Fact. 13, 130 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene A5N73_13410, sequence comparisons
Rhodococcus hoagii
Engineering
Protein Variants
Commentary
Organism
additional information
deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 by a PCR-targeted gene disruption method results in a mutant strain that accumulates up to 0.58 mg/ml 1,4-androstadiene-3,17-dione in the culture medium when 0.2% cholesterol is used as the carbon source. The mutant also accumulates 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (DELTA1,4-BNC). 1,4-Androstadiene-3,17-dione in strain USA-18DELTAB8 preferentially imposes a feedback inhibition on the enzymes involved in the side chain oxidation of DELTA1,4-BNC, but not DELTA4-BNC. Mutant phenotypes, overview
Rhodococcus hoagii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii
-
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii 103S
-
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii
-
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii 103S
-
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rhodococcus hoagii
A0A060CNE1
i.e. Rhodococcus hoagii
-
Rhodococcus hoagii 103S
A0A060CNE1
i.e. Rhodococcus hoagii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii 103S
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii 103S
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
3-ketosteroid 9alpha-hydroxylase
-
Rhodococcus hoagii
A5N73_13410
-
Rhodococcus hoagii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
in vivo assay at
Rhodococcus hoagii
Cloned(Commentary) (protein specific)
Commentary
Organism
gene A5N73_13410, sequence comparisons
Rhodococcus hoagii
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 by a PCR-targeted gene disruption method results in a mutant strain that accumulates up to 0.58 mg/ml 1,4-androstadiene-3,17-dione in the culture medium when 0.2% cholesterol is used as the carbon source. The mutant also accumulates 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (DELTA1,4-BNC). 1,4-Androstadiene-3,17-dione in strain USA-18DELTAB8 preferentially imposes a feedback inhibition on the enzymes involved in the side chain oxidation of DELTA1,4-BNC, but not DELTA4-BNC. Mutant phenotypes, overview
Rhodococcus hoagii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii
-
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii 103S
-
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii
-
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Rhodococcus hoagii 103S
-
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii 103S
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
745698
Rhodococcus hoagii 103S
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
in vivo assay at
Rhodococcus hoagii
General Information
General Information
Commentary
Organism
malfunction
deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione
Rhodococcus hoagii
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione
Rhodococcus hoagii
Other publictions for EC 1.14.15.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745648
Guevara
Functional characterization o ...
Rhodococcus ruber, Rhodococcus ruber Chol-4
J. Steroid Biochem. Mol. Biol.
172
176-187
2017
-
-
1
-
1
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
22
1
11
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
22
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
746503
Zhang
Efficient 9alpha-hydroxy-4-an ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12
SpringerPlus
5
1207
2016
-
-
2
-
1
-
5
-
-
-
-
8
-
4
-
-
1
-
-
-
1
1
10
3
4
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
1
-
-
5
-
-
-
-
-
8
-
-
-
1
-
-
1
1
10
3
2
-
-
-
2
-
-
-
-
1
1
-
-
-
743221
Yao
Characterization and engineer ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01, Mycolicibacterium neoaurum ATCC 25795
Metab. Eng.
24
181-191
2014
-
-
1
-
1
-
-
-
-
1
-
3
-
4
-
-
-
-
-
-
-
-
3
-
6
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
744058
Petrusma
3-Ketosteroid 9alpha-hydroxyl ...
Mycolicibacterium smegmatis, Rhodococcus erythropolis, Rhodococcus rhodochrous, Rhodococcus jostii, Mycobacterium tuberculosis, Mycolicibacterium smegmatis mc2 155, Rhodococcus erythropolis SQ1, Mycobacterium tuberculosis H37Rv, Rhodococcus rhodochrous DSM 43269
Antonie van Leeuwenhoek
106
157-172
2014
-
2
5
-
1
-
-
-
-
8
-
22
-
20
-
-
-
8
-
-
-
-
28
7
22
-
-
-
-
-
-
-
15
-
-
-
-
2
5
15
-
1
-
-
-
-
-
-
8
-
22
-
-
-
-
-
-
-
-
28
7
-
-
-
-
-
-
-
-
-
29
29
-
-
-
745308
Penfield
Substrate specificities and c ...
Rhodococcus rhodochrous, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Rhodococcus rhodochrous DSM 43269
J. Biol. Chem.
289
25523-25536
2014
-
-
2
5
-
-
4
30
-
-
-
6
-
25
-
-
2
3
-
-
-
-
44
3
16
3
-
-
29
3
-
-
-
3
-
-
-
-
2
-
5
-
-
-
6
3
30
-
-
-
6
-
-
-
2
-
-
-
-
44
3
3
-
-
29
3
-
-
-
-
6
6
-
27
27
745698
Yeh
Deletion of the gene encoding ...
Rhodococcus hoagii, Rhodococcus hoagii 103S
Microb. Cell Fact.
13
130
2014
-
-
1
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
717737
Petrusma
Structural features in the Ksh ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
194
115-121
2012
-
-
5
-
25
-
-
4
-
2
-
3
-
22
-
-
3
-
-
-
-
-
74
-
14
-
-
-
-
2
-
-
4
-
-
-
-
-
5
4
-
25
-
-
-
-
4
-
2
-
3
-
-
-
3
-
-
-
-
74
-
-
-
-
-
2
-
-
-
-
2
2
-
-
-
717735
Petrusma
Multiplicity of 3-ketosteroid- ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
193
3931-3940
2011
-
-
8
-
5
-
-
-
-
-
-
4
-
27
-
-
8
-
-
3
-
-
75
-
19
-
-
-
-
3
-
-
5
-
-
-
-
-
8
5
-
5
-
-
-
-
-
-
-
-
4
-
-
-
8
-
3
-
-
75
-
-
-
-
-
3
-
-
-
5
11
11
5
-
-
717854
Capyk
Activity of 3-ketosteroid 9alp ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
286
40717-40724
2011
-
-
-
-
-
-
-
21
-
-
-
2
-
9
-
-
-
-
-
-
-
-
32
2
6
2
-
-
19
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
21
-
-
-
2
-
-
-
-
-
-
-
-
32
2
2
-
-
19
2
-
-
-
-
3
3
-
19
19
713784
Wei
A new steroid-transforming str ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01
Appl. Biochem. Biotechnol.
162
1446-1456
2010
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
716250
Hu
3-Ketosteroid 9alpha-hydroxyla ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Mol. Microbiol.
75
107-121
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
181
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
717500
Fan
Cloning, heterologous expressi ...
Mycobacterium sp., Mycobacterium sp. NwIB-01
Chin. J. Biotechnol.
25
2014-2021
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
9
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717784
Capyk
Characterization of 3-ketoster ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
284
9937-9946
2009
-
-
1
1
-
-
-
3
-
-
-
-
-
7
-
-
1
-
-
-
-
-
2
-
2
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
717119
Van Der Geize
Characterization of a second R ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Appl. Environ. Microbiol.
74
7197-7203
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
8
-
4
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
717118
Andor
Generation of useful insertion ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Appl. Environ. Microbiol.
72
6554-6559
2006
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718125
Van Der Geize
Molecular and functional chara ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Mol. Microbiol.
45
1007-1018
2002
-
-
-
-
2
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
8
-
6
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-