KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic parameters for KshAB, overview | Mycobacterium tuberculosis | |
0.003 | - |
3-oxo-23,24-bisnorchol-4-en-22-oic acid | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.003 | - |
3-oxo-23,24-bisnorcholesta-4-ene-22-oate | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.0068 | - |
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.0068 | - |
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.017 | - |
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.017 | - |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.024 | - |
4-androstene-3,17-dione | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.024 | - |
4-androstene-3,17-dione | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.07 | - |
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.07 | - |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.09 | - |
O2 | apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.1 | 1 | androsta-1,4-diene-3,17-dione | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.11 | - |
androsta-1,4-diene-3,17-dione | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Mycobacterium tuberculosis | - |
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Mycobacterium tuberculosis | - |
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis | P71875 | gene kshA; the enzyme harbors a Rieske oxygenase, encoded by kshA | - |
Mycobacterium tuberculosis | P9WJ93 | gene kshB; the enzyme harbors a reductase, encoded by kshB | - |
Mycobacterium tuberculosis H37Rv | P71875 | gene kshA; the enzyme harbors a Rieske oxygenase, encoded by kshA | - |
Mycobacterium tuberculosis H37Rv | P9WJ93 | gene kshB; the enzyme harbors a reductase, encoded by kshB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis H37Rv | 9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis H37Rv | 9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis H37Rv | 9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | - |
Mycobacterium tuberculosis | ? | - |
r | |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis H37Rv | 9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxy-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
4-androstene-3,17-dione + O2 + NADH + H+ | - |
Mycobacterium tuberculosis | ? + H2O + NAD+ | - |
? | |
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
additional information | substrate specificity, overview. KshAB also transforms CoA thioester steroids | Mycobacterium tuberculosis | ? | - |
? | |
additional information | substrate specificity, overview. KshAB also transforms CoA thioester steroids | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme comprises a Rieske oxygenase, encoded by kshA, and a reductase, encoded by kshB | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid 9alpha-hydroxylase | - |
Mycobacterium tuberculosis |
KshAB | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
4-androstene-3,17-dione | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.07 | - |
4-androstene-3,17-dione | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.08 | - |
3-oxo-23,24-bisnorchol-4-en-22-oic acid | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.08 | - |
3-oxo-23,24-bisnorcholesta-4-ene-22-oate | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.25 | - |
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.25 | - |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.61 | - |
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.61 | - |
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
0.8 | - |
androsta-1,4-diene-3,17-dione | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
0.8 | - |
androsta-1,4-diene-3,17-dione | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
2.5 | - |
O2 | apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
2.7 | - |
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
2.7 | - |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Fe-S center | Rieske-type Fe-S cluster, Rieske monooxygenase | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme substrate binding pocket analysis using molecular modeling, overview. Modeling of substrates into KshA crystal structure using KshA structure PDB 2ZYL | Mycobacterium tuberculosis |
physiological function | the enzyme plays a role of cholesterol catabolism, overview | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.917 | - |
4-androstene-3,17-dione | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
3 | - |
4-androstene-3,17-dione | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
3.5 | - |
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
3.57 | - |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
7.27 | - |
androsta-1,4-diene-3,17-dione | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
7.6 | - |
androsta-1,4-diene-3,17-dione | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
26.67 | - |
3-oxo-23,24-bisnorcholesta-4-ene-22-oate | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
29 | - |
O2 | apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
30 | - |
3-oxo-23,24-bisnorchol-4-en-22-oic acid | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
89.71 | - |
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
90 | - |
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis | |
158.8 | - |
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA | pH 7.0, 22°C, KshAB | Mycobacterium tuberculosis | |
160 | - |
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA | in 0.1 M potassium phosphate, pH 7.0, at 22°C | Mycobacterium tuberculosis |