BRENDA - Enzyme Database
show all sequences of 1.14.15.30

Activity of 3-ketosteroid 9alpha-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis

Capyk, J.; Casabon, I.; Gruninger, R.; Strynadka, N.; Eltis, L.; J. Biol. Chem. 286, 40717-40724 (2011)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetic parameters for KshAB, overview
Mycobacterium tuberculosis
0.003
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.003
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.0068
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.0068
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.017
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.017
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.024
-
4-androstene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.024
-
4-androstene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.07
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.07
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.09
-
O2
apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.1
1
androsta-1,4-diene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.11
-
androsta-1,4-diene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Mycobacterium tuberculosis
-
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Mycobacterium tuberculosis
-
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis
P71875
gene kshA; the enzyme harbors a Rieske oxygenase, encoded by kshA
-
Mycobacterium tuberculosis
P9WJ93
gene kshB; the enzyme harbors a reductase, encoded by kshB
-
Mycobacterium tuberculosis H37Rv
P71875
gene kshA; the enzyme harbors a Rieske oxygenase, encoded by kshA
-
Mycobacterium tuberculosis H37Rv
P9WJ93
gene kshB; the enzyme harbors a reductase, encoded by kshB
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
717854
Mycobacterium tuberculosis
?
-
-
-
r
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
-
717854
Mycobacterium tuberculosis
? + H2O + NAD+
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
additional information
substrate specificity, overview. KshAB also transforms CoA thioester steroids
717854
Mycobacterium tuberculosis
?
-
-
-
?
additional information
substrate specificity, overview. KshAB also transforms CoA thioester steroids
717854
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the enzyme comprises a Rieske oxygenase, encoded by kshA, and a reductase, encoded by kshB
Mycobacterium tuberculosis
Synonyms
Synonyms
Commentary
Organism
3-ketosteroid 9alpha-hydroxylase
-
Mycobacterium tuberculosis
KshAB
-
Mycobacterium tuberculosis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Mycobacterium tuberculosis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.07
-
4-androstene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.07
-
4-androstene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.08
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.08
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.25
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.25
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.61
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.61
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.8
-
androsta-1,4-diene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.8
-
androsta-1,4-diene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
2.5
-
O2
apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
2.7
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
2.7
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Mycobacterium tuberculosis
Cofactor
Cofactor
Commentary
Organism
Structure
Fe-S center
Rieske-type Fe-S cluster, Rieske monooxygenase
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Fe-S center
Rieske-type Fe-S cluster, Rieske monooxygenase
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetic parameters for KshAB, overview
Mycobacterium tuberculosis
0.003
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.003
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.0068
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.0068
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.017
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.017
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.024
-
4-androstene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.024
-
4-androstene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.07
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.07
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.09
-
O2
apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.1
1
androsta-1,4-diene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.11
-
androsta-1,4-diene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Mycobacterium tuberculosis
-
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
Mycobacterium tuberculosis
-
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholest-4-en-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
717854
Mycobacterium tuberculosis
?
-
-
-
r
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis H37Rv
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxy-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
-
717854
Mycobacterium tuberculosis
? + H2O + NAD+
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717854
Mycobacterium tuberculosis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
additional information
substrate specificity, overview. KshAB also transforms CoA thioester steroids
717854
Mycobacterium tuberculosis
?
-
-
-
?
additional information
substrate specificity, overview. KshAB also transforms CoA thioester steroids
717854
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the enzyme comprises a Rieske oxygenase, encoded by kshA, and a reductase, encoded by kshB
Mycobacterium tuberculosis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.07
-
4-androstene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.07
-
4-androstene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.08
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.08
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.25
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.25
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.61
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.61
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
0.8
-
androsta-1,4-diene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
0.8
-
androsta-1,4-diene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
2.5
-
O2
apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
2.7
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
2.7
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Mycobacterium tuberculosis
General Information
General Information
Commentary
Organism
additional information
enzyme substrate binding pocket analysis using molecular modeling, overview. Modeling of substrates into KshA crystal structure using KshA structure PDB 2ZYL
Mycobacterium tuberculosis
physiological function
the enzyme plays a role of cholesterol catabolism, overview
Mycobacterium tuberculosis
General Information (protein specific)
General Information
Commentary
Organism
additional information
enzyme substrate binding pocket analysis using molecular modeling, overview. Modeling of substrates into KshA crystal structure using KshA structure PDB 2ZYL
Mycobacterium tuberculosis
physiological function
the enzyme plays a role of cholesterol catabolism, overview
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.917
-
4-androstene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
3
-
4-androstene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
3.5
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
3.57
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
7.27
-
androsta-1,4-diene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
7.6
-
androsta-1,4-diene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
26.67
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
29
-
O2
apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
30
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
89.71
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
90
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
158.8
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
160
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.917
-
4-androstene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
3
-
4-androstene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
3.5
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
3.57
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
7.27
-
androsta-1,4-diene-3,17-dione
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
7.6
-
androsta-1,4-diene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
26.67
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
29
-
O2
apparent Km value, in the presence of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA, in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
30
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
89.71
-
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
90
-
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
158.8
-
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
pH 7.0, 22C, KshAB
Mycobacterium tuberculosis
160
-
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22C
Mycobacterium tuberculosis
Other publictions for EC 1.14.15.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745648
Guevara
Functional characterization o ...
Rhodococcus ruber, Rhodococcus ruber Chol-4
J. Steroid Biochem. Mol. Biol.
172
176-187
2017
-
-
1
-
1
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
22
1
11
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
22
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
746503
Zhang
Efficient 9alpha-hydroxy-4-an ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12
SpringerPlus
5
1207
2016
-
-
2
-
1
-
5
-
-
-
-
8
-
4
-
-
1
-
-
-
1
1
10
3
4
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
1
-
-
5
-
-
-
-
-
8
-
-
-
1
-
-
1
1
10
3
2
-
-
-
2
-
-
-
-
1
1
-
-
-
743221
Yao
Characterization and engineer ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01, Mycolicibacterium neoaurum ATCC 25795
Metab. Eng.
24
181-191
2014
-
-
1
-
1
-
-
-
-
1
-
3
-
4
-
-
-
-
-
-
-
-
3
-
6
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
744058
Petrusma
3-Ketosteroid 9alpha-hydroxyl ...
Mycolicibacterium smegmatis, Rhodococcus erythropolis, Rhodococcus rhodochrous, Rhodococcus jostii, Mycobacterium tuberculosis, Mycolicibacterium smegmatis mc2 155, Rhodococcus erythropolis SQ1, Mycobacterium tuberculosis H37Rv, Rhodococcus rhodochrous DSM 43269
Antonie van Leeuwenhoek
106
157-172
2014
-
2
5
-
1
-
-
-
-
8
-
22
-
20
-
-
-
8
-
-
-
-
28
7
22
-
-
-
-
-
-
-
15
-
-
-
-
2
5
15
-
1
-
-
-
-
-
-
8
-
22
-
-
-
-
-
-
-
-
28
7
-
-
-
-
-
-
-
-
-
29
29
-
-
-
745308
Penfield
Substrate specificities and c ...
Rhodococcus rhodochrous, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Rhodococcus rhodochrous DSM 43269
J. Biol. Chem.
289
25523-25536
2014
-
-
2
5
-
-
4
30
-
-
-
6
-
25
-
-
2
3
-
-
-
-
44
3
16
3
-
-
29
3
-
-
-
3
-
-
-
-
2
-
5
-
-
-
6
3
30
-
-
-
6
-
-
-
2
-
-
-
-
44
3
3
-
-
29
3
-
-
-
-
6
6
-
27
27
745698
Yeh
Deletion of the gene encoding ...
Rhodococcus hoagii, Rhodococcus hoagii 103S
Microb. Cell Fact.
13
130
2014
-
-
1
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
717737
Petrusma
Structural features in the Ksh ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
194
115-121
2012
-
-
5
-
25
-
-
4
-
2
-
3
-
22
-
-
3
-
-
-
-
-
74
-
14
-
-
-
-
2
-
-
4
-
-
-
-
-
5
4
-
25
-
-
-
-
4
-
2
-
3
-
-
-
3
-
-
-
-
74
-
-
-
-
-
2
-
-
-
-
2
2
-
-
-
717735
Petrusma
Multiplicity of 3-ketosteroid- ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
193
3931-3940
2011
-
-
8
-
5
-
-
-
-
-
-
4
-
27
-
-
8
-
-
3
-
-
75
-
19
-
-
-
-
3
-
-
5
-
-
-
-
-
8
5
-
5
-
-
-
-
-
-
-
-
4
-
-
-
8
-
3
-
-
75
-
-
-
-
-
3
-
-
-
5
11
11
5
-
-
717854
Capyk
Activity of 3-ketosteroid 9alp ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
286
40717-40724
2011
-
-
-
-
-
-
-
21
-
-
-
2
-
9
-
-
-
-
-
-
-
-
32
2
6
2
-
-
19
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
21
-
-
-
2
-
-
-
-
-
-
-
-
32
2
2
-
-
19
2
-
-
-
-
3
3
-
19
19
713784
Wei
A new steroid-transforming str ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01
Appl. Biochem. Biotechnol.
162
1446-1456
2010
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
716250
Hu
3-Ketosteroid 9alpha-hydroxyla ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Mol. Microbiol.
75
107-121
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
181
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
717500
Fan
Cloning, heterologous expressi ...
Mycobacterium sp., Mycobacterium sp. NwIB-01
Chin. J. Biotechnol.
25
2014-2021
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
9
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717784
Capyk
Characterization of 3-ketoster ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
284
9937-9946
2009
-
-
1
1
-
-
-
3
-
-
-
-
-
7
-
-
1
-
-
-
-
-
2
-
2
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
717119
Van Der Geize
Characterization of a second R ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Appl. Environ. Microbiol.
74
7197-7203
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
8
-
4
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
717118
Andor
Generation of useful insertion ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Appl. Environ. Microbiol.
72
6554-6559
2006
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718125
Van Der Geize
Molecular and functional chara ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Mol. Microbiol.
45
1007-1018
2002
-
-
-
-
2
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
8
-
6
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-