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Literature summary for 1.14.15.3 extracted from

  • Xie, M.; Alonso, H.; Roujeinikova, A.
    An improved procedure for the purification of catalytically active alkane hydroxylase from Pseudomonas putida GPo1 (2011), Appl. Biochem. Biotechnol., 165, 823-831.
    View publication on PubMed
Search for more literature for 1.14.15.3

Application

Application Comment Organism
environmental protection the enzyme has a tremendous biotechnological potential as a biocatalyst and promising application in the bioremediation of oil-contaminated environments Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
gene alkB, expression of C-terminally StrepII-tagged AlkB in membranes of Escherichia coli strain NovaBlue Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 1-octene pH 7.4, 30°C, recombinant enzyme Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane AlkB is an integral membrane monooxygenase Pseudomonas putida 16020
-

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q9WWW6 gene alkB
-
Pseudomonas putida GPo1 / ATCC29347 Q9WWW6 gene alkB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally StrepII-tagged AlkB from membranes of Escherichia coli strain NovaBlue by detergent solubilization, and Strep-tag affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-octene + reduced rubredoxin + O2 epoxidation reaction catalysed by AlkB Pseudomonas putida 1,2-epoxyoctane + oxidized rubredoxin + H2O
-
 ?
1-octene + reduced rubredoxin + O2 epoxidation reaction catalysed by AlkB Pseudomonas putida GPo1 / ATCC29347 1,2-epoxyoctane + oxidized rubredoxin + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 44000-46000, recombinant StrepII-tagged AlkB, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
AlkB
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
rubredoxin endogenous, recombinantly expressed in Escherichia coli TG10 cells Pseudomonas putida