Application | Comment | Organism |
---|---|---|
environmental protection | the enzyme has a tremendous biotechnological potential as a biocatalyst and promising application in the bioremediation of oil-contaminated environments | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
gene alkB, expression of C-terminally StrepII-tagged AlkB in membranes of Escherichia coli strain NovaBlue | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
1-octene | pH 7.4, 30°C, recombinant enzyme | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | AlkB is an integral membrane monooxygenase | Pseudomonas putida | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q9WWW6 | gene alkB | - |
Pseudomonas putida GPo1 / ATCC29347 | Q9WWW6 | gene alkB | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally StrepII-tagged AlkB from membranes of Escherichia coli strain NovaBlue by detergent solubilization, and Strep-tag affinity chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-octene + reduced rubredoxin + O2 | epoxidation reaction catalysed by AlkB | Pseudomonas putida | 1,2-epoxyoctane + oxidized rubredoxin + H2O | - |
? | |
1-octene + reduced rubredoxin + O2 | epoxidation reaction catalysed by AlkB | Pseudomonas putida GPo1 / ATCC29347 | 1,2-epoxyoctane + oxidized rubredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000-46000, recombinant StrepII-tagged AlkB, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
rubredoxin | endogenous, recombinantly expressed in Escherichia coli TG10 cells | Pseudomonas putida |