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Literature summary for 1.14.15.3 extracted from

  • Ruettiger, R.T.; Olson, S.T.; Boyer, R.F.; Coon, M.J.
    Identification of the omega-hydroxylase of Pseudomonas oleovorans as a nonheme iron protein requiring phospholipid for catalytic activity (1974), Biochem. Biophys. Res. Commun., 57, 1011-1017.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid enhances enzyme activity Pseudomonas oleovorans

General Stability

General Stability Organism
treatment with 0.1 M EDTA and 0.1 M dithionite reduces activity to 10% Pseudomonas oleovorans
treatment with sodium cholate and subsequent precipitation with ammonium sulfate reduces activity to 40% Pseudomonas oleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe non-heme iron protein Pseudomonas oleovorans
Fe 1 atom Fe per 42000 g/mol peptide Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Pseudomonas oleovorans
2000000
-
-
Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
n-alkanol + NAD(P)+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
addition of dilauroylglyceryl-3-phosphorylcholine restores initial activity after treatment with sodium cholate and subsequent precipitation with ammonium sulfate Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.59
-
-
Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fatty acid + NAD(P)H + H+ + O2
-
Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
?
n-alkane + NAD(P)H + H+ + O2
-
Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
?
n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Pseudomonas oleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay conditions Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans