Cloned (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure | Mycolicibacterium smegmatis |
to 2.26 A resolution | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
W83Y | mutation to the corresponding residue of isoform CYP125A4. Mutation reduces the size of the binding pocket, reducing the ability to bind 7alpha-hydroxylated substrates | Mycolicibacterium smegmatis |
Y83W | mutation to the corresponding residue of isoform CYP125A3. Mutation increases the size of the binding pocket, increasing the ability to bind 7alpha-hydroxylated substrates. Enzyme shows activity toward 4-cholesten-3-one, albeit with sigmoidal kinetics | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
7alpha-hydroxycholest-4-en-3-one | wild-type, pH 7.4, temperature not specified in the publication | Mycolicibacterium smegmatis | |
0.0051 | - |
7alpha-hydroxycholest-4-en-3-one | mutant Y87W, pH 7.4, temperature not specified in the publication | Mycolicibacterium smegmatis | |
0.0241 | - |
cholest-4-en-3-one | mutant W87Y, pH 7.4, temperature not specified in the publication | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0QY38 | - |
- |
Mycolicibacterium smegmatis | A0R4Y3 | - |
- |
Mycolicibacterium smegmatis ATCC 700084 | A0QY38 | - |
- |
Mycolicibacterium smegmatis ATCC 700084 | A0R4Y3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7alpha-hydroxycholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycolicibacterium smegmatis | (25S)-7alphahydroxy-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
7alpha-hydroxycholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycolicibacterium smegmatis ATCC 700084 | (25S)-7alphahydroxy-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
cholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycolicibacterium smegmatis | (25S)-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
cholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycolicibacterium smegmatis ATCC 700084 | (25S)-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
cholest-5-en-3beta-ol + 6 reduced [2Fe-2S] ferredoxin + 3 O2 | - |
Mycolicibacterium smegmatis | (25S)-3beta-hydroxycholest-5-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? | |
cholest-5-en-3beta-ol + 6 reduced [2Fe-2S] ferredoxin + 3 O2 | - |
Mycolicibacterium smegmatis ATCC 700084 | (25S)-3beta-hydroxycholest-5-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP125A3 | - |
Mycolicibacterium smegmatis |
CYP125A4 | - |
Mycolicibacterium smegmatis |
Organism | Comment | Expression |
---|---|---|
Mycolicibacterium smegmatis | expression is not upregulated in response to cholesterol | additional information |