Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodococcus jostii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47200 | - |
x * 47200, calculated from amino acid sequence | Rhodococcus jostii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Rhodococcus jostii |
Subunits | Comment | Organism |
---|---|---|
? | x * 47200, calculated from amino acid sequence | Rhodococcus jostii |
Synonyms | Comment | Organism |
---|---|---|
CYP125 | - |
Rhodococcus jostii |
CYP125A1 | - |
Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Rhodococcus jostii | |
NADH | - |
Rhodococcus jostii |
General Information | Comment | Organism |
---|---|---|
metabolism | CYP125 performs an obligate first step in cholesterol degradation. CYP125 is involved in steroid C26-carboxylic acid formation, catalysing the oxidation of C26 either to the corresponding carboxylic acid or to an intermediate state | Rhodococcus jostii |
physiological function | cyp125 is essential for Rhodococcus jostii strain RHA1 to grow on 3-hydroxysterols such as cholesterol, but not on 3-oxo sterol derivatives | Rhodococcus jostii |