Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.29 extracted from

  • Rosloniec, K.; Wilbrink, M.; Capyk, J.; Mohn, W.; Ostendorf, M.; Van Der Geize, R.; Dijkhuizen, L.; Eltis, L.
    Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1 (2009), Mol. Microbiol., 74, 1031-1043.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus jostii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47200
-
x * 47200, calculated from amino acid sequence Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Rhodococcus jostii

Subunits

Subunits Comment Organism
? x * 47200, calculated from amino acid sequence Rhodococcus jostii

Synonyms

Synonyms Comment Organism
CYP125
-
Rhodococcus jostii
CYP125A1
-
Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
heme
-
Rhodococcus jostii
NADH
-
Rhodococcus jostii

General Information

General Information Comment Organism
metabolism CYP125 performs an obligate first step in cholesterol degradation. CYP125 is involved in steroid C26-carboxylic acid formation, catalysing the oxidation of C26 either to the corresponding carboxylic acid or to an intermediate state Rhodococcus jostii
physiological function cyp125 is essential for Rhodococcus jostii strain RHA1 to grow on 3-hydroxysterols such as cholesterol, but not on 3-oxo sterol derivatives Rhodococcus jostii