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Literature summary for 1.14.15.29 extracted from

  • Johnston, J.B.; Ouellet, H.; Ortiz de Montellano, P.R.
    Functional redundancy of steroid C26-monooxygenase activity in Mycobacterium tuberculosis revealed by biochemical and genetic analyses (2010), J. Biol. Chem., 285, 36352-36360.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0107
-
 cholesterol pH and temperature not specified in the publication Mycobacterium tuberculosis
0.0208
-
 cholest-4-en-3-one pH and temperature not specified in the publication Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis CDC 1551
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycobacterium tuberculosis (25S)-26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O CYP125A1 generates oxidized sterols of the (25S)-26-hydroxy configuration ?
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycobacterium tuberculosis H37Rv (25S)-26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O CYP125A1 generates oxidized sterols of the (25S)-26-hydroxy configuration ?
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycobacterium tuberculosis CDC 1551 (25S)-26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O CYP125A1 generates oxidized sterols of the (25S)-26-hydroxy configuration ?
cholesterol + reduced ferredoxin [iron-sulfur] cluster + O2
-
 Mycobacterium tuberculosis 26-hydroxycholesterol + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
cholesterol + reduced ferredoxin [iron-sulfur] cluster + O2
-
 Mycobacterium tuberculosis H37Rv 26-hydroxycholesterol + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
cholesterol + reduced ferredoxin [iron-sulfur] cluster + O2
-
 Mycobacterium tuberculosis CDC 1551 26-hydroxycholesterol + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP125A1
-
 Mycobacterium tuberculosis
steroid C26-monooxygenase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.47
-
 cholesterol pH and temperature not specified in the publication Mycobacterium tuberculosis
2.92
-
 cholest-4-en-3-one pH and temperature not specified in the publication Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Mycobacterium tuberculosis