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Literature summary for 1.14.15.29 extracted from

  • Ouellet, H.; Kells, P.; Ortiz De Montellano, P.; Podust, L.
    Reverse type I inhibitor of Mycobacterium tuberculosis CYP125A1 (2011), Bioorg. Med. Chem. Lett., 21, 332-337.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 2.0 M ammonium sulfate, 0.1 M bis-Tris (pH 5.5) and 0.5 M NaCl (truncated enzyme C429L in complex with inhibitor) or 0.1 M ammonium acetate, 0.1 M bis-Tris (pH 5.5) and 17% PEG 10000, or 2.0 M ammonium sulfate and 0.1 M Tris HCl pH 8.5 (truncated enzyme C429L without any ligand) Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C429L the modification affect neither the binding nor the catalytic properties of the enzyme Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
alpha-[(4-methylcyclohexyl)carbonyl amino]-N-4-pyridinyl-1H-indole-3-propanamide LP10, 30% inhibition at 0.2 mM Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPP1
-
-
Mycobacterium tuberculosis H37Rv P9WPP1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycobacterium tuberculosis 26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycobacterium tuberculosis H37Rv 26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP125A1
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Mycobacterium tuberculosis