General Stability | Organism |
---|---|
after separation of the solubilized enzyme by anion exchange chromatography, the stability of xylene monooxygenase is drastically reduced | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas putida | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P21395 | encoded by TOL plasmid | - |
Purification (Comment) | Organism |
---|---|
activity can be solubilized by detergents, octyl-beta-glucopyranoside and 3-[(3-cholamidopropyl)dimethyiammonio]-l-propanesulfonate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
m-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Pseudomonas putida | 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
o-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Pseudomonas putida | 2-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
p-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Pseudomonas putida | 4-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Pseudomonas putida | benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas putida |