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Literature summary for 1.14.15.26 extracted from

  • Shaw, J.; Harayama, S.
    Characterization in vitro of the hydroxylase component of xylene monooxygenase, the first enzyme of the TOL-plasmid-encoded pathway for the mineralization of toluene and xylenes (1995), J. Ferment. Bioeng., 79, 195-199 .
No PubMed abstract available

General Stability

General Stability Organism
after separation of the solubilized enzyme by anion exchange chromatography, the stability of xylene monooxygenase is drastically reduced Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Pseudomonas putida 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P21395 encoded by TOL plasmid
-

Purification (Commentary)

Purification (Comment) Organism
activity can be solubilized by detergents, octyl-beta-glucopyranoside and 3-[(3-cholamidopropyl)dimethyiammonio]-l-propanesulfonate Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
m-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Pseudomonas putida 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
o-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Pseudomonas putida 2-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
p-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Pseudomonas putida 4-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Pseudomonas putida benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pseudomonas putida