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Literature summary for 1.14.15.24 extracted from

  • Tian, L.; Magallanes-Lundback, M.; Musetti, V.; DellaPenna, D.
    Functional analysis of beta- and epsilon-ring carotenoid hydroxylases in Arabidopsis (2003), Plant Cell, 15, 1320-1332.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.24

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme is a non-heme di-iron protein Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LTG0 ecotypes Columbia and Wassilewskija
-
Arabidopsis thaliana Q9SZZ8 ecotypes Columbia and Wassilewskija
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-carotene + reduced acceptor + H+ + O2
-
 Arabidopsis thaliana zeinoxanthin + acceptor + H2O
-
 ?
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Arabidopsis thaliana cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
 Arabidopsis thaliana zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Arabidopsis thaliana zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
additional information although beta-hydroxylase 1 is active toward beta-rings, it can also add a hydroxyl group to epsilon-rings in vitro, although at much lower efficiency Arabidopsis thaliana ?
-
 ?
additional information although beta-hydroxylase 2 is most active toward beta-rings, it can also add a hydroxyl group to epsilon-rings in vitro, although at much lower efficiency Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-carotene hydroxylase
-
 Arabidopsis thaliana
beta-hydroxylases 1
-
 Arabidopsis thaliana
beta-hydroxylases 2
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction in the homozygous b1 b2 double mutant both beta-hydroxylase activities are eliminated, but beta-ring-hydroxylated xanthophylls (L, V, and N) still are synthesized, albeit at lower levels than in ecotype Wassilewskija or in either single mutant. Xanthophylls N and V are reduced by 90 and 65%, respectively, and there is a 30% increase in xanthophyll L relative to ecotype Wassilewskija. As with the single beta-hydroxylase mutants, beta-carotene is unchanged relative to the wild type and beta-cryptoxanthin does not accumulate Arabidopsis thaliana
physiological function beta-hydroxylase 1 and 2 are functionally redundant in vivo Arabidopsis thaliana