Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Capsicum annuum |
Protein Variants | Comment | Organism |
---|---|---|
H171A | inactive | Capsicum annuum |
H176A | inactive | Capsicum annuum |
H184A | inactive | Capsicum annuum |
H187A | inactive | Capsicum annuum |
H188A | inactive | Capsicum annuum |
H245A | inactive | Capsicum annuum |
H250A | inactive | Capsicum annuum |
H274A | inactive | Capsicum annuum |
H275A | inactive | Capsicum annuum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 5% residual activity at 1 mM | Capsicum annuum | |
cytochrome c | addition of cytochrome c to the incubation medium inhibits hydroxylase activity | Capsicum annuum | |
o-phenanthroline | 10% residual activity at 1 mM | Capsicum annuum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | 26000 | SDS-PAGE | Capsicum annuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | O49814 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Talon nickel-affinity resin column chromatography, and Mono Q column chromatography | Capsicum annuum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Capsicum annuum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase | Capsicum annuum | beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 | the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase | Capsicum annuum | zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase | Capsicum annuum | zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carotenoid hydroxylase 2 | - |
Capsicum annuum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | the hydroxylase is strictly dependent upon reduced ferredoxin which cannot be replaced by NADPH | Capsicum annuum |