Literature summary for 1.14.15.22 extracted from

Sawada, N.; Sakaki, T.; Yoneda, S.; Kusudo, T.; Shinkyo, R.; Ohta, M.; Inouye, K.
Conversion of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 by Streptomyces griseolus cytochrome P450SU-1 (2004), Biochem. Biophys. Res. Commun., 320, 156-164.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces griseolus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00054	-	calciol	vitamin D3 25-hydroxylation, pH 7.4, 30°C	Streptomyces griseolus
0.00059	-	vitamin D2	vitamin D2 25-hydroxylation, pH 7.4, 30°C	Streptomyces griseolus
0.00091	-	calcidiol	25-hydroxyvitamin D3 1alpha-hydroxylation, pH 7.4, 30°C	Streptomyces griseolus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseolus	P18326	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Streptomyces griseolus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
calcidiol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2	-	Streptomyces griseolus	calcitriol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
calciol + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	-	Streptomyces griseolus	calcidiol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
vitamin D2 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	-	Streptomyces griseolus	25-hydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP105A1	-	Streptomyces griseolus
cytochrome P450SU-1	-	Streptomyces griseolus
vitamin D3 dihydroxylase	-	Streptomyces griseolus

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Release 2023.1 (January 2023)