Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.20 extracted from

  • Zhang, X.; Migita, C.; Sato, M.; Sasahara, M.; Yoshida, T.
    Protein expressed by the ho2 gene of the cyanobacterium Synechocystis sp. PCC 6803 is a true heme oxygenase: Properties of the heme and enzyme complex (2005), FEBS J., 272, 1012-1022.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
x * 28500, calculated from amino acid sequence Synechocystis sp.
29000
-
x * 29000, SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Synechocystis sp.
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-75 gel filtration , DE-52 column chromatography, and hydroxyapatite column chromatography Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoheme + reduced ferredoxin [iron-sulfur] cluster + H2O2 + H+
-
Synechocystis sp. verdoheme + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
Synechocystis sp. biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Synechocystis sp.
? x * 28500, calculated from amino acid sequence Synechocystis sp.

Synonyms

Synonyms Comment Organism
heme oxygenase
-
Synechocystis sp.
HO-2 isoform Synechocystis sp.