Literature summary for 1.14.15.18 extracted from

Lobaugh, B.; Almond, J.R.; Drezner, M.K.
Measurement of 25-hydroxyvitamin D 1-alpha-hydroxylase activity in mammalian kidney (1986), Methods Enzymol., 123, 159-167.

Search for more literature for 1.14.15.18

Activating Compound

Activating Compound	Comment	Organism	Structure
malate	supports 1alpha-hydroxylation as electron donor	Mammalia
succinate	supports 1alpha-hydroxylation as electron donor	Mammalia

Inhibitors

Inhibitors	Comment	Organism	Structure
antimycin A	-	Mammalia
CN-	-	Mammalia
CO	-	Mammalia
cyanide	-	Mammalia
dinitrophenol	-	Mammalia
Glutethimide	-	Mammalia
Metyrapone	-	Mammalia
Natural inhibitors	vitamin D binding protein	Mammalia
rotenone	-	Mammalia

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	almost any other Krebs cycle substrate supports 1-alpha-hydroxylation	Mammalia

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	ferredoxin, ferredoxin reductase and cytochrome P-450	Mammalia

Organism

Organism	UniProt	Comment	Textmining
Mammalia	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O	25-hydroxyvitamin D3 1alpha-hydroxylase is a cytochrome P-450-dependent mixed-function oxidase	Mammalia	a
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O	ferredoxin, ferredoxin reductase and cytochrome P-450	Mammalia	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Mammalia	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
calcidiol + NADPH + O2	highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol	Mammalia	calcitriol + NADP+ + H2O	calcitriol is identical with 1,25-dihydroxycholecalciferol	?

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Mammalia

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Release 2023.1 (January 2023)