Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme can be reconstituted with the 3 components: cytochrome P-450D1alpha, NADPH-renodoxin reductase and renodoxin | Bos taurus |
49000 | - |
cytochrome P-450D1alpha, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of cytochrome P-450D1alpha | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | enzyme can be reconstituted with the 3 components: cytochrome P-450D1alpha, NADPH-renodoxin reductase and renodoxin | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000082 | - |
purified cytochrome P-450 component of 1alpha-hydroxylase, reconstituted system | Bos taurus |
Storage Stability | Organism |
---|---|
-80°C, in the dark, stable for at least 3 months, cytochrome P-450D1alpha | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Bos taurus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bos taurus |