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Literature summary for 1.14.15.18 extracted from

  • Hiwatashi, A.; Nishii, Y.; Ichikawa, Y.
    Purification of cytochrome P-450D1 alpha (25-hydroxyvitamin D3-1 alpha-hydroxylase) of bovine kidney mitochondria (1982), Biochem. Biophys. Res. Commun., 105, 320-327.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
enzyme can be reconstituted with the 3 components: cytochrome P-450D1alpha, NADPH-renodoxin reductase and renodoxin Bos taurus
49000
-
cytochrome P-450D1alpha, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of cytochrome P-450D1alpha Bos taurus

Reaction

Reaction Comment Organism Reaction ID
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O enzyme can be reconstituted with the 3 components: cytochrome P-450D1alpha, NADPH-renodoxin reductase and renodoxin Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000082
-
purified cytochrome P-450 component of 1alpha-hydroxylase, reconstituted system Bos taurus

Storage Stability

Storage Stability Organism
-80°C, in the dark, stable for at least 3 months, cytochrome P-450D1alpha Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Bos taurus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Bos taurus