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Literature summary for 1.14.15.18 extracted from

  • Kulkoski, J.A.; Ghazarian, J.G.
    Purification and characterization of the ferredoxin component of 25-hydroxycholecalciferol 1 alpha-hydroxylase (1979), Biochem. J., 177, 673-678.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.18

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12500
-
 ferredoxin component, gel filtration Gallus gallus
53000
-
 MW of ferredoxin component, SDS-PAGE, gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
25-hydroxycholecalciferol + NADPH + O2 Gallus gallus
-
 calcitriol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of ferredoxin component Gallus gallus

Reaction

Reaction Comment Organism Reaction ID
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O ferredoxin, ferredoxin reductase and cytochrome P-450 Gallus gallus
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.687
-
 ferredoxin activity, reduction of cytochrome c Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
25-hydroxycholecalciferol + NADPH + O2
-
 Gallus gallus calcitriol + NADP+ + H2O
-
 ?
calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Gallus gallus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Gallus gallus