Activating Compound | Comment | Organism | Structure |
---|---|---|---|
malate | supports 1alpha-hydroxylation as electron donor | Gallus gallus | |
succinate | supports 1alpha-hydroxylation as electron donor | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,25-Dihydroxycholecalciferol | calcitriol | Gallus gallus | |
25-hydroxydihydrotachysterol3 | - |
Gallus gallus | |
antimycin A | - |
Gallus gallus | |
CO | 50% O2/50% CO2 incubation atmosphere | Gallus gallus | |
cyanide | - |
Gallus gallus | |
dinitrophenol | - |
Gallus gallus | |
diphenyl-p-phenylenediamine | - |
Gallus gallus | |
Metyrapone | - |
Gallus gallus | |
oligomycin | - |
Gallus gallus | |
p-chloromercuribenzoate | - |
Gallus gallus | |
phosphate | - |
Gallus gallus | |
sucrose | hypertonic | Gallus gallus | |
Tris-chloride buffer | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
25-hydroxycholecalciferol | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Gallus gallus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxycholecalciferol + NADPH + O2 | Gallus gallus | central role in calcium regulation | calcitriol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00125 | - |
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxycholecalciferol + NADPH + O2 | central role in calcium regulation | Gallus gallus | calcitriol + NADP+ + H2O | - |
? | |
calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Gallus gallus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
additional information | not: cholecalciferol, dihydrotachysterol | Gallus gallus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.7 | about 50% of activity maximum at pH 7.1 and pH 7.7 | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Gallus gallus |