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Literature summary for 1.14.15.17 extracted from

  • Pruzinska, A.; Tanner, G.; Anders, I.; Roca, M.; Hortensteiner, S.
    Chlorophyll breakdown: pheophorbide a oxygenase is a Rieske-type iron-sulfur protein, encoded by the accelerated cell death 1 gene (2003), Proc. Natl. Acad. Sci. USA, 100, 15259-15264.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana
gene PaO, functional recombinant expression in Escherichia coli strain C43 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Arabidopsis thaliana
0.006
-
 pheophorbide a pH 7.0, 24°C, recombinant enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast envelope PaO is localized in the chloroplast inner envelope Arabidopsis thaliana 9941
-
gerontoplast the enzyme contains a chloroplast transit peptide Arabidopsis thaliana 34400
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe Rieske-type iron-sulfur cluster-containing protein Arabidopsis thaliana
Iron-sulfur cluster AtPaO is a Rieske-type iron–sulfur cluster-containing enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana PaO expression is correlated positively with senescence. The in vivo function of PaO is the degradation of pheide a during senescence ?
-
 ?
pheophorbide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Arabidopsis thaliana
-
 red chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q9FYC2 gene PaO
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
additional information PaO activity is found only during senescence Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PaO expression is correlated positively with senescence. The in vivo function of PaO is the degradation of pheide a during senescence Arabidopsis thaliana ?
-
 ?
pheophorbide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Arabidopsis thaliana red chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Synonyms

Synonyms Comment Organism
ACD1
-
 Arabidopsis thaliana
AtPaO
-
 Arabidopsis thaliana
PAO
-
 Arabidopsis thaliana
pheophorbide a oxygenase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Arabidopsis thaliana
NADPH
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
evolution AtPaO is a Rieske-type iron–sulfur cluster-containing enzyme that is identical to Arabidopsis accelerated cell death 1 and homologous to lethal leaf spot 1 (LLS1) of maize Arabidopsis thaliana
metabolism enzyme PaO seems to be a key regulator of chlorophyll catabolism Arabidopsis thaliana
physiological function chlorophyll breakdown during senescence is an integral part of plant development and leads to the accumulation of colorless catabolites. The loss of green pigment is due to an oxygenolytic opening of the porphyrin macrocycle of pheophorbide (pheide) a followed by a reduction to yield a fluorescent chlorophyll catabolite. This step is comprised of the interaction of two enzymes, pheide a oxygenase (PaO) and red chl catabolite reductase. Senescence-related activity of PaO Arabidopsis thaliana