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Literature summary for 1.14.15.16 extracted from
- Kinetic analysis of human CYP24A1 metabolism of vitamin D via the C24-oxidation pathway (2014), FEBS J., 281, 3280-3296.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CYP24A1, functional recombinant His-tagged enzyme expression in Escherichia coli membranes | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | reconstitution of the metabolism of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and the intermediates of the C24-oxidation pathway in a phospholipid-vesicle system. Changes to the phospholipid concentration do not affect the kinetic parameters for the metabolism of 1,25(OH)2D3 by CYP24A1, indicating that it is the concentration of substrates in the membrane phase that determines their rate of metabolism | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | substrate kinetics, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | CYP24A1 is a membrane-bound hemoprotein | Homo sapiens | 16020 | - |
| membrane | CYP24A1 is a membrane-bound hemoprotein | Rattus norvegicus | 16020 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, about, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Homo sapiens | - |
calcitroic acid + 2 oxidized adrenodoxin + H2O | - |
? |  |
| 23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Rattus norvegicus | - |
calcitroic acid + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Homo sapiens | - |
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Rattus norvegicus | - |
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Homo sapiens | - |
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Rattus norvegicus | - |
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Homo sapiens | - |
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | Rattus norvegicus | - |
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 | Homo sapiens | - |
secalciferol + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2 | Homo sapiens | - |
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2 | Rattus norvegicus | - |
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitriol + 2 reduced adrenodoxin + 2 H+ + O2 | Homo sapiens | - |
calcitetrol + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitriol + 2 reduced adrenodoxin + 2 H+ + O2 | Rattus norvegicus | - |
calcitetrol + 2 oxidized adrenodoxin + H2O | - |
? | |
| additional information | Homo sapiens | calcidiol, the precursor to calcitriol, is also a substrate of CYP24A1 and is metabolized through similar pathways | ? | - |
? | |
| additional information | Rattus norvegicus | the precursor to calcitriol, calcidiol, is also a substrate of CYP24A1 and is metabolized through similar pathways | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q07973 | gene CYP24A1 | - |
| Rattus norvegicus | Q09128 | gene CYP24A1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant active His-tagged enzyme from Escherichia coli membranes by nickel affinity and hydrophobic interaction chromatography and dialysis, adrenodoxin-affinity chromatography is not successful | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | calcitroic acid + 2 oxidized adrenodoxin + H2O | - |
? | |
| 23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Rattus norvegicus | calcitroic acid + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | 23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Rattus norvegicus | 23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | 24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Rattus norvegicus | 24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | 24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| 24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Rattus norvegicus | 24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | secalciferol + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | 24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Rattus norvegicus | 24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitriol + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | calcitetrol + 2 oxidized adrenodoxin + H2O | - |
? | |
| calcitriol + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Rattus norvegicus | calcitetrol + 2 oxidized adrenodoxin + H2O | - |
? | |
| additional information | calcidiol, the precursor to calcitriol, is also a substrate of CYP24A1 and is metabolized through similar pathways | Homo sapiens | ? | - |
? | |
| additional information | the precursor to calcitriol, calcidiol, is also a substrate of CYP24A1 and is metabolized through similar pathways | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme activity measurements with reconstituted enzyme in phospholipid vesicles | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, about, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP24A1 | - |
Homo sapiens |
| CYP24A1 | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| adrenodoxin | - |
Homo sapiens | |
| adrenodoxin | - |
Rattus norvegicus | |
| heme | CYP24A1 is a membrane-bound hemoprotein | Homo sapiens | |
| heme | CYP24A1 is a membrane-bound hemoprotein | Rattus norvegicus | |
| NADPH | - |
Homo sapiens | |
| NADPH | - |
Rattus norvegicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | CYP24A1 is the multicatalytic cytochrome P450 responsible for the catabolism of vitamin D via the C23- and C24-oxidation pathways. CYP24A1 is a membrane-bound hemoprotein which utilizes the electrons from NADPH via a transport chain comprising adrenodoxin and adrenodoxin reductase. Inactivation of 1,25(OH)2D3 by CYP24A1 via the C24-oxidation pathway | Rattus norvegicus |
| physiological function | CYP24A1 is the multicatalytic cytochrome P450 responsible for the catabolism of vitamin D via the C23- and C24-oxidation pathways. CYP24A1 is a membrane-bound hemoprotein which utilizes the electrons from NADPH via a transport chain comprising adrenodoxin and adrenodoxin reductase. Inactivation of 1,25(OH)2D3 by CYP24A1 via the C24-oxidation pathway, overview | Homo sapiens |
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