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Literature summary for 1.14.15.16 extracted from
- Bioengineering anabolic vitamin D-25-hydroxylase activity into the human vitamin D catabolic enzyme, cytochrome P450 CYP24A1, by a V391L mutation (2011), J. Biol. Chem., 286, 28729-28737.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in chinese hamster lung fibroblast cells (V79-4 cells) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A326G | the mutant converts 1alpha-hydroxyvitamin-D3 into 1alpha,25-dihydroxyvitamin-D3-26,23-lactone | Homo sapiens |
| V391L | the mutation converts the enzyme from a catabolic 1alpha,25-dihydroxyvitamin-D3-24-hydroxylase into an anabolic 1alpha-hydroxyvitamin-D3-25-hydroxylase. The mutant enzyme retains its basal ability to catabolize 1alpha,25-dihydroxyvitamin D3 via C24 hydroxylation | Homo sapiens |
| V391L/A326G | the mutant enzyme continues to form 1alpha,25-dihydroxyvitamin D3 from 1alpha-hydroxyvitamin-D3, but this initial product is diverted via the C23 hydroxylation pathway into 1alpha,25-dihydroxyvitamin-D3-26,23-lactone | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | Homo sapiens | - |
calcitroic acid + NADP+ + ? | - |
? |  |
| 25-hydroxyvitamin D3 + NADPH + H+ + O2 | Homo sapiens | - |
24,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
| additional information | Homo sapiens | no activity with 1alpha-hydroxyvitamin D3 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | calcitroic acid + NADP+ + ? | - |
? | |
| 25-hydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | 24,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
| additional information | no activity with 1alpha-hydroxyvitamin D3 | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP24A1 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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