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Literature summary for 1.14.15.15 extracted from
- Enzymatic characteristics of CO-sensitive 26-hydroxylase system for 5beta-cholestane-3alpha,7alpha 12alpha-triol in rat-liver mitochondria and its intramitochondrial localization (1973), Eur. J. Biochem., 40, 607-617.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CO | at a CO:O2 ratio of 0.75 hydroxylation reaction is undetectable | Rattus norvegicus |  |
| dicoumarol | 10% inhibition at 0.05 mM | Rattus norvegicus | |
| p-chloromercuriphenyl sulfonate | 80% inhibition at 0.1 mM | Rattus norvegicus | |
| Phenyl isocyanide | 70% inhibition at 0.1 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | 0.02 | O2 | - |
Rattus norvegicus | |
| 0.06 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | inner membrane-matrix | Rattus norvegicus | 31966 | - |
| additional information | only the mitochondrial hydroxylase system is specific for C-26 position, the microsomal system is unspecific for C-26 position but rather more active for other adjacent positions | Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | Rattus norvegicus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | isocitrate can act as electron donor | Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | mitochondrial enzyme is specific for 26-hydroxylation whereas the reaction with the microsomal enzyme hydroxylates at position 23, 24, 25 and 26 | Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | possible function of a cytochrome-P450-like entity in the intramitochondrial 26-hydroxylase system | Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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