Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, free enzyme using 1% (w/v) PEGMME 2000, 0.1 M Na cacodylate (pH 6.8), and 0.9 M succinic acid, and enzyme in complex with phytanic acid using 21% (w/v) PEG 4000, 0.1 M Bis-Tris (pH 5.5), and 0.2 M calcium acetate | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
15-methylpalmitic acid | at pH 7.4 and 25°C | Mycobacterium tuberculosis | |
0.032 | - |
geranylgeraniol | at pH 7.4 and 25°C | Mycobacterium tuberculosis | |
0.036 | - |
farnesol | at pH 7.4 and 25°C | Mycobacterium tuberculosis | |
0.054 | - |
phytanic acid | at pH 7.4 and 25°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48700 | - |
x * 48700, calculated from amino acid sequence | Mycobacterium tuberculosis |
50000 | - |
x * 50000, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | Mycobacterium tuberculosis | - |
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | Mycobacterium tuberculosis H37Rv | - |
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPP3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPP3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis | ? | - |
? | |
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis | an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis H37Rv | an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | best substrate | Mycobacterium tuberculosis | ? | - |
? | |
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | best substrate | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis | ? | - |
? | |
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
palmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | the enzyme oxidizes the omega-1,omega-2, and omega-position of palmitic acid | Mycobacterium tuberculosis | ? | - |
? | |
phytanic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Mycobacterium tuberculosis |
? | x * 48700, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CYP124 | - |
Mycobacterium tuberculosis |