Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme A356T, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.5), 0.1 M MgCl2, 12% (w/v) polyethylene glycol 3350 | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
A356T | mutant enzyme A356T crystallizes under several PEG-3350 conditions, with an optimum pH around 6 and a relatively low PEG concentration (12-15% (w/v)). The wild-type protein does not crystallize under these conditions, while the protein of the A356T mutant crystallizes readily and forms large, thin plates | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclo(L-leucyl-L-leucyl) + reduced ferredoxin + O2 | Bacillus subtilis | - |
pulcherriminic acid + oxidized ferredoxin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34926 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Resource Q column chromatography, and Superose-12 gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenylimidazole + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
2,5-di-tert-butylhydroquinone + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
2,5-di-tert-butylquinone + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
2-phenylimidazole + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
4-phenylimidazole + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
cyclo(L-alanyl-L-alanyl) + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
cyclo(L-leucyl-L-leucyl) + reduced ferredoxin + O2 | - |
Bacillus subtilis | pulcherriminic acid + oxidized ferredoxin + H2O | - |
? | |
cyclo(L-leucyl-L-phenylalanyl) + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
cyclo(L-leucyl-L-prolyl) + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
cyclo(L-leucyl-L-tryptophanyl) + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
cyclo(L-methionyl-L-methionyl) + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
cyclo(L-valyl-L-valyl) + reduced ferredoxin + O2 | - |
Bacillus subtilis | ? + oxidized ferredoxin + H2O | - |
? | |
additional information | the enzyme does not use dimethylpyrazine and tetramethylpyrazine as substrates | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cyclo-L-leucyl-L-leucyl dipeptide oxidase | - |
Bacillus subtilis |
CYP134A1 | - |
Bacillus subtilis |
CypX | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bacillus subtilis |