Literature summary for 1.14.15.11 extracted from

Yao, Q.; Ma, L.; Liu, L.; Ikeda, H.; Fushinobu, S.; Li, S.; Xu, L.H.
Hydroxylation of Compactin (ML-236B) by CYP105D7 (SAV_7469) from Streptomyces avermitilis (2017), J. Microbiol. Biotechnol., 27, 956-964 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escheirchia coli	Streptomyces avermitilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular docking of compactin, compactin is directly hydrogen bonded with residues Thr79, Arg81, Leu178, Thr391, and Ile392 and its C6 position is proximate to the heme group. Molecular docking of diclofenac	Streptomyces avermitilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.039	-	Compactin	pH 7.4, 30°C	Streptomyces avermitilis
0.19	-	diclofenac	pH 7.4, 30°C	Streptomyces avermitilis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces avermitilis	Q825I8	-	-
Streptomyces avermitilis DSM 46492	Q825I8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
compactin + reduced ferredoxin + O2	-	Streptomyces avermitilis	pravastatin + oxidized ferredoxin + H2O	-	?
compactin + reduced ferredoxin + O2	-	Streptomyces avermitilis DSM 46492	pravastatin + oxidized ferredoxin + H2O	-	?
compactin + reduced putidaredoxin + O2	-	Streptomyces avermitilis	pravastatin + oxidized putidaredoxin + H2O	-	?
compactin + reduced putidaredoxin + O2	-	Streptomyces avermitilis DSM 46492	pravastatin + oxidized putidaredoxin + H2O	-	?
diclofenac + reduced putidaredoxin + O2	-	Streptomyces avermitilis	4'-hydroxydiclofenac + oxidized putidaredoxin + H2O	-	?
diclofenac + reduced putidaredoxin + O2	-	Streptomyces avermitilis DSM 46492	4'-hydroxydiclofenac + oxidized putidaredoxin + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE, recombinant protein	Streptomyces avermitilis

Synonyms

Synonyms	Comment	Organism
CYP105D7	-	Streptomyces avermitilis
SAV_7469	-	Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.018	-	Compactin	pH 7.4, 30°C	Streptomyces avermitilis
0.36	-	diclofenac	pH 7.4, 30°C	Streptomyces avermitilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.48	-	Compactin	pH 7.4, 30°C	Streptomyces avermitilis
1.9	-	diclofenac	pH 7.4, 30°C	Streptomyces avermitilis

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Release 2023.1 (January 2023)