Literature summary for 1.14.15.1 extracted from

Skinner, S.; Liu, W.; Hiruma, Y.; Timmer, M.; Blok, A.; Hass, M.; Ubbink, M.
Delicate conformational balance of the redox enzyme cytochrome P450cam (2015), Proc. Natl. Acad. Sci. USA, 112, 9022-9027 .

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Protein Variants

Protein Variants	Comment	Organism
E195C/A199C/C334A	site-directed mutagenesis, substrate and cofactor binding of the mutant compared to the wild-type, overview	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-camphor + reduced putidaredoxin + O2	Pseudomonas putida	-	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	P00183	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-camphor + reduced putidaredoxin + O2	-	Pseudomonas putida	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?

Synonyms

Synonyms	Comment	Organism
cytochrome p450cam	-	Pseudomonas putida
P450cam	-	Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Pseudomonas putida
putidaredoxin	cofactor putidaredxin-bound enzyme structure analysis using crystal structure, PDB ID 4JWS	Pseudomonas putida

General Information

General Information	Comment	Organism
additional information	cofactor putidaredxin-bound and substrate-bound wild-type and E195C/A199C mutant enzymes, camphor-free, cyanide-bound P450cam, and apoenzyme structure analysis, structure analysis using crystal structure, PDB IDs 4JWS and 3L63. Cyanide and camphor can bind simultaneously in the active site	Pseudomonas putida

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Release 2023.1 (January 2023)