Literature summary for 1.14.15.1 extracted from

Eichler, A.; Gricman, �.; Herter, S.; Kelly, P.; Turner, N.; Pleiss, J.; Flitsch, S.
Enantioselective benzylic hydroxylation catalysed by P450 monooxygenases characterisation of a P450cam mutant library and molecular modelling (2016), ChemBioChem, 17, 426-432 .

Search for more literature for 1.14.15.1

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of wild-type enzyme, mutant P450cam[Tyr96Phe]-RhFRed, and other enzyme mutants in Escherichia coli strain BL21(DE3)	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
L244F/V247L	site-directed mutagenesis, the mutant exhibits moderate to high R-selectivity toward ethylmethylbenzene substrates and shows a narrow width of the binding pocket	Pseudomonas putida
L244N/V247L	site-directed mutagenesis, the mutant displays the highest S-selectivity toward substrates 1-ethyl-2-methylbenzene and 1-ethyl-3-methylbenzene, and low R-selectivity toward 1-ethyl-4-methylbenzene and shows a narrow width of the binding pocket	Pseudomonas putida
M184V/T185F	site-directed mutagenesis, the mutation introduces changes above the heme plane, prefers S-orientation of 1-ethyl-4-methylbenzene in the binding pocket of mutant, enantioselectivities of 1-ethyl-2-methylbenzene and 1-ethyl-3-methylbenzene are similar to the wild-type enzyme	Pseudomonas putida
additional information	enantioselectivity of a library of active-site mutants of chimeric P450cam-RhFRed towards the benzylic hydroxylation of structurally related regioisomers of ethylmethylbenzene, computational molecular modeling, overview	Pseudomonas putida
Y96F	site-directed mutagenesis	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-camphor + reduced putidaredoxin + O2	Pseudomonas putida	-	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	P00183	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-camphor + reduced putidaredoxin + O2	-	Pseudomonas putida	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?
1-ethyl-2-methylbenzene + reduced putidaredoxin + O2	the S-enantiomer is preferred by the wild-type enzyme	Pseudomonas putida	? + oxidized putidaredoxin + H2O	-	?
1-ethyl-3-methylbenzene + reduced putidaredoxin + O2	the S-enantiomer is preferred by the wild-type enzyme	Pseudomonas putida	? + oxidized putidaredoxin + H2O	-	?
1-ethyl-4-methylbenzene + reduced putidaredoxin + O2	the S-enantiomer is preferred by the wild-type enzyme	Pseudomonas putida	? + oxidized putidaredoxin + H2O	-	?
additional information	enzyme P450cam produces benzyl alcohols with only moderate enantioselectivities, enantioselective benzylic hydroxylation catalysed by P450 monooxygenases, enantioselectivity of a library of active-site mutants of chimeric P450cam-RhFRed towards the benzylic hydroxylation of structurally related regioisomers of ethylmethylbenzene, molecular dynamics simulations and computational molecular modelling, overview	Pseudomonas putida	?	-	?

Synonyms

Synonyms	Comment	Organism
CYP101	-	Pseudomonas putida
P450cam	-	Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Pseudomonas putida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Pseudomonas putida
NADH	-	Pseudomonas putida
putidaredoxin	-	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)