Literature summary for 1.14.15.1 extracted from

Kim, D.; Ortiz de Montellano, P.R.
Tricistronic overexpression of cytochrome P450cam, putidaredoxin, and putidaredoxin reductase provides a useful cell-based catalytic system (2009), Biotechnol. Lett., 31, 1427-1431.

Search for more literature for 1.14.15.1

Application

Application	Comment	Organism
biotechnology	bioengineered Escherichia coli cells possess a heterologous self-sufficient P450 catalytic system that may have advantages in terms of low cost and high yield for the production of fine chemicals	Pseudomonas putida

Cloned(Commentary)

Cloned (Comment)	Organism
functional expression in Escherichia coli DH5alpha of tricistronic constructs consisting of P450cam encoded by the first cistron and the auxiliary proteins, putidaredoxin and putidaredoxin reductase by the second and the third	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	x * 47000, SDS-PAGE/Coomassie staining	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(1R)-(+)-camphor + O2 + reduced putidaredoxin	-	Pseudomonas putida	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?
(4S)-limonene + putidaredoxin + O2	the 7-position is the major site of hydroxylation by P450cam	Pseudomonas putida	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 47000, SDS-PAGE/Coomassie staining	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
cytochrome p450cam	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
putidaredoxin	-	Pseudomonas putida

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Release 2023.1 (January 2023)