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Literature summary for 1.14.15.1 extracted from

  • Ryan, J.; Clark, D.
    P450cam biocatalysis in surfactant-stabilized two-phase emulsions (2008), Biotechnol. Bioeng., 99, 1311-1319.
    View publication on PubMed
Search for more literature for 1.14.15.1

Activating Compound

Activating Compound Comment Organism Structure
bis(2-ethylhexyl) sulfosuccinate improves the initial activity of P450cam in two-phase emulsions with initial camphor concentrations of 5-15 mM. P450cam is activated in the surfactant-free emulsions, and addition of bis(2-ethylhexyl) sulfosuccinate sodium salt improves the activity even further, at least over the range of camphor concentrations for which initial rates are readily measurable in all media. The largest rate enhancement is 4.5fold. Nearly 50times more product is formed in the surfactant-stabilized emulsions than is achieved in aqueous buffer Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
plasmids transformed into Escherichia coli Bl21(DE3) competent cells Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
additional information emulsions formed by strongly agitating the mixture with a vortexer denatured protein, P450cam has a complete loss of activity when incubated under these conditions for more than 5 min prior to the initiation of the reaction Pseudomonas putida

Organic Solvent Stability

Organic Solvent Comment Organism
hexane P450cam is stable and retains 80% of its initial activity after 1 h in a hexane/water emulsion at agitation speeds of less than 250 rpm Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by anion exchange, hydrophobic interaction and gel filtration Pseudomonas putida

Storage Stability

Storage Stability Organism
-80°C Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
(R)-exo-5-hydroxycamphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida 2,5-diketocamphane + oxidized putidaredoxin + H2O
-
 ?

Synonyms

Synonyms Comment Organism
P450cam
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome m optimal ratio of putidaredoxin reductase, putidaredoxin, cytochrome m is 1:10:2 Pseudomonas putida
putidaredoxin optimal ratio of putidaredoxin reductase, putidaredoxin, cytochrome m is 1:10:2 Pseudomonas putida