Literature summary for 1.14.15.1 extracted from

Celik, A.; Speight, R.E.; Turner, N.J.
Identification of broad specificity P450CAM variants by primary screening against indole as substrate (2005), Chem. Commun. (Camb. ), 2005, 3652-3654.

Search for more literature for 1.14.15.1

Protein Variants

Protein Variants	Comment	Organism
Y96A	site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole	Pseudomonas putida
Y96C	site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole	Pseudomonas putida
Y96G	site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole	Pseudomonas putida
Y96M	site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole	Pseudomonas putida
Y96Q	site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole	Pseudomonas putida
Y96S	site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole	Pseudomonas putida
Y96T	site-directed mutagenesis, the mutant gains the ability to hydroxylate indole to 3-hydroxyindole	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-camphor + O2 + reduced putidaredoxin	Pseudomonas putida	-	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-camphor + O2 + reduced putidaredoxin	-	Pseudomonas putida	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?
indole + O2 + reduced putidaredoxin	no substrate of the wild-type enzyme, but a good substrate for Y96 mutants, mutant screening, overview	Pseudomonas putida	3-hydroxyindole + oxidized putidaredoxin + H2O	3-hydroxyindole undergoes spontaneous air oxidation to produce the insoluble dye indigo	?

Synonyms

Synonyms	Comment	Organism
P450cam	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Pseudomonas putida
putidaredoxin	-	Pseudomonas putida

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Release 2023.1 (January 2023)