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Literature summary for 1.14.15.1 extracted from

  • Purdy, M.M.; Koo, L.S.; Montellano, P.R.; Klinman, J.P.
    Mechanism of O2 activation by cytochrome P450cam studied by isotope effects and transient state kinetics (2006), Biochemistry, 45, 15793-15806.
    View publication on PubMed
Search for more literature for 1.14.15.1

Inhibitors

Inhibitors Comment Organism Structure
additional information increasing viscosity of the assay mixture, e.g. by addition of glucose or sucrose, reduces kcat Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information equilibrium isotope effect on O2 binding, isotope effects and transient-state kinetics, electron transfer kinetics, steady-state kinetics, recombinant His6-tagged enzyme, overview Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a cytochrome P450 monooxygenase Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O mechanism of O2 activation: binding of O2 to ferrous P450cam to yield the ferric-superoxo form, oxyP450cam, followed by an irreversible, long-range electron transfer from putidaredoxin to reduce the oxyP450cam, camphor is bound to all enzyme forms, overview Pseudomonas putida
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 r

Synonyms

Synonyms Comment Organism
cytochrome p450cam
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39
-
 (+)-Camphor pH 7.9, 25°C, recombinant His6-tagged enzyme Pseudomonas putida
47
-
 (+)-Camphor pH 6.9, 25°C, recombinant His6-tagged enzyme Pseudomonas putida
51
-
 (+)-Camphor pH 7.4, 25°C, recombinant His6-tagged enzyme Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH profile Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
putidaredoxin electron transfer from putidaredoxin to the ferric-superoxo enzyme form, overview Pseudomonas putida