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Literature summary for 1.14.15.1 extracted from

  • Nickerson, D.P.; Wong, L.L.
    The dimerization of Pseudomonas putida cytochrome P450cam: practical consequences and engineering of a monomeric enzyme (1997), Protein Eng., 10, 1357-1361.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C334A identical with the wild-type monomer in terms of optical spectra, camphor-binding and turnover Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
Accession Number PC00183
-

Storage Stability

Storage Stability Organism
4°C, monomeric cytochrome P450cam can be stored at low protein concentrations for several days without appreciable accumulation of the dimer Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + putidaredoxin + O2
-
Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Subunits

Subunits Comment Organism
More cytochrome P450cam dimerizes via the formation of an intermolecular disulfide bond Pseudomonas putida