Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Nicotiana sylvestris |
Protein Variants | Comment | Organism |
---|---|---|
E375K | loss of catalytic activity | Nicotiana sylvestris |
additional information | analysis of polymorphic variations of the CYP82E2 orthologs in Nicotiana sylvestris and various Nicotiana tabacum genotypes | Nicotiana sylvestris |
W422L | loss of catalytic activity | Nicotiana sylvestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
Nicotine | pH 7.1, 23°C | Nicotiana sylvestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana sylvestris | A7KMF3 | isoform CYP82E2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | highly active in senescing leaf | Nicotiana sylvestris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Nicotiana sylvestris | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |