Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | Nicotiana tabacum | - |
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | isoform CYP82E4v1 is responsible for the conversion of nicotine to nornicotine | ? | |
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | Nicotiana tabacum | nornicotine is a secondary tobacco alkaloid that is produced by the N-demethylation of nicotine. Nornicotine production and accumulation in tobacco are undesirable because nornicotine serves as the precursor in the synthesis of the carcinogen N-nitrosonornicotine during the curing and processing of tobacco | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q38Q86 | CYP82E4 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | cured leaf | Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Nicotiana tabacum | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | isoform CYP82E4v1 is responsible for the conversion of nicotine to nornicotine | ? | |
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | nornicotine is a secondary tobacco alkaloid that is produced by the N-demethylation of nicotine. Nornicotine production and accumulation in tobacco are undesirable because nornicotine serves as the precursor in the synthesis of the carcinogen N-nitrosonornicotine during the curing and processing of tobacco | Nicotiana tabacum | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Nicotiana tabacum |