Literature summary for 1.14.14.99 extracted from

Wriessnegger, T.; Moser, S.; Emmerstorfer-Augustin, A.; Leitner, E.; Mueller, M.; Kaluzna, I.; Schuermann, M.; Mink, D.; Pichler, H.
Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions (2016), Fungal Genet. Biol., 89, 114-125 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition	Mentha x piperita

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Pichia pastoris. The enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition	Mentha x piperita

Cloned (Comment)

Organism

overexpression in Pichia pastoris. The enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition

Mentha x piperita

Organism

Organism	UniProt	Comment	Textmining
Mentha x piperita	Q9XHE7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2	-	Mentha x piperita	(-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
(-)-limonene-3-hydroxylase	-	Mentha x piperita
PM17	-	Mentha x piperita

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P-450	a heme-thiolate protein (P-450)	Mentha x piperita
NADPH-hemoprotein reductase	A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._	Mentha x piperita