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Literature summary for 1.14.14.98 extracted from

  • Takemura, T.; Ikezawa, N.; Iwasa, K.; Sato, F.
    Molecular cloning and characterization of a cytochrome P450 in sanguinarine biosynthesis from Eschscholzia californica cells (2013), Phytochemistry, 91, 100-108.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Eschscholzia californica

Inhibitors

Inhibitors Comment Organism Structure
ketoconazole less than 2% activity at 0.025 mM Eschscholzia californica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00645
-
 6-hydroxyprotopine in 50 mM HEPES/NaOH (pH 7.6), at 30°C Eschscholzia californica
0.0087
-
 6-hydroxyallocryptopine in 50 mM HEPES/NaOH (pH 7.6), at 30°C Eschscholzia californica

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Eschscholzia californica
-
-

Organism

Organism UniProt Comment Textmining
Eschscholzia californica F2Z9C1 california poppy
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13-oxoprotopine + [reduced NADPH-hemoprotein reductase] + O2
-
 Eschscholzia californica ?
-
 ?
allocryptopine + [reduced NADPH-hemoprotein reductase] + O2
-
 Eschscholzia californica 6-hydroxyallocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
corycavine + [reduced NADPH-hemoprotein reductase] + O2
-
 Eschscholzia californica corynoloxine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information no activity with protopine-N-oxide, reticuline, scoulerine, and N-methyl stylopine Eschscholzia californica ?
-
 ?
protopine + [reduced NADPH-hemoprotein reductase] + O2 i.e. dihydrosanguinarine Eschscholzia californica 6-hydroxyprotopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP82N2v2
-
 Eschscholzia californica
P6H
-
 Eschscholzia californica
protopine 6-hydroxylase
-
 Eschscholzia californica