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Literature summary for 1.14.14.94 extracted from

  • Christmas, P.; Ursino, S.R.; Fox, J.W.; Soberman, R.J.
    Expression of the CYP4F3 gene. Tissue-specific splicing and alternative promoters generate high and low Km forms of leukotriene B4.omega-hydroxylase (1999), J. Biol. Chem., 274, 21191-21199.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
chromosomal localization 19p Homo sapiens
CYP4F3 gene contains 14 exons and 13 introns Homo sapiens
expression in transfected COS-7 cells Homo sapiens
tissue-specific expression is regulated by alternative promoter usage and mutually exclusive exon splicing, exon 3 and 4 Homo sapiens
two isoforms of CYP4F3, CYP4F3A expressed in neutrophils and CYP4F3B expressed in fetal and adult liver Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
leukotriene B4 recombinant isoform CYP4F3A, expressed in COS-7 cells Homo sapiens
0.105
-
leukotriene B4 recombinant isoform CYP4F3B, expressed in COS-7 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
recombinant isoforms CYP4F3A and CYP4F3B expressed in COS-7 cells, western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens inactivation of leukotriene B4, a mediator of inflammation (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver isoform CYP4F3B Homo sapiens
-
neutrophil isoform CYP4F3A Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 inactivation of leukotriene B4, a mediator of inflammation Homo sapiens (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate Homo sapiens 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP4F3
-
Homo sapiens
cytochrome P450 4F3
-
Homo sapiens