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Literature summary for 1.14.14.92 extracted from

  • Faber, B.W.; van Gorcom, R.F.; Duine, J.A.
    Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger (2001), Arch. Biochem. Biophys., 394, 245-254.
    View publication on PubMed
Search for more literature for 1.14.14.92

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 benzoate
-
 Aspergillus niger
0.086
-
 3-Fluorobenzoate
-
 Aspergillus niger
0.097
-
 2-Fluorobenzoate
-
 Aspergillus niger
0.127
-
 3-chlorobenzoate
-
 Aspergillus niger
0.189
-
 3-Methoxybenzoate
-
 Aspergillus niger
0.28
-
 2-Hydroxybenzoate
-
 Aspergillus niger
0.31
-
 2-Chlorobenzoate
-
 Aspergillus niger
0.673
-
 3-methylbenzoate
-
 Aspergillus niger
1.6
-
 2-Methylbenzoate
-
 Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, benzoate-agarose Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2-chloro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2-fluorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2-fluoro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2-methylbenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2-methyl-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-chlorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-chloro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-fluorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-fluoro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-methoxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-methoxy-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-methylbenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-methyl-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
nicotinate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger ?
-
 ?
picolinate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.33
-
 2-Chlorobenzoate
-
 Aspergillus niger
3.67
-
 2-Methylbenzoate
-
 Aspergillus niger
3.83
-
 picolinate
-
 Aspergillus niger
4
-
 nicotinate
-
 Aspergillus niger
4
-
 3-Methoxybenzoate
-
 Aspergillus niger
4
-
 benzoate turnover rate in microsomes Aspergillus niger
4.17
-
 2-Hydroxybenzoate
-
 Aspergillus niger
4.33
-
 2-Fluorobenzoate
-
 Aspergillus niger
4.5
-
 benzoate
-
 Aspergillus niger
4.5
-
 3-hydroxybenzoate
-
 Aspergillus niger
4.83
-
 3-Fluorobenzoate
-
 Aspergillus niger
6.17
-
 3-chlorobenzoate
-
 Aspergillus niger
6.17
-
 3-methylbenzoate
-
 Aspergillus niger